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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Recombinant Monoclonal CD46 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | IP | Flow Cyt | WB | |
---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Tested |
Rabbit | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rabbit | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rabbit | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info - | Notes - |
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.(Microbial infection) Acts as a receptor for cultured Measles virus.(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).
Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, MIC10, CD46, MCP
Rabbit Recombinant Monoclonal CD46 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, MIC10, CD46, MCP
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR4014
Affinity purification Protein A
Blue Ice
+4°C
Do Not Freeze
ab229446 is the carrier-free version of ab108307.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Beyond its complement regulatory role CD46 acts in immune response modulation and reproduction. It forms a part of a protein complex that involves members such as decay-accelerating factor and complement receptor 1. CD46 also engages in intracellular signaling that affects T cell function and differentiation. In the reproductive context it participates in sperm-oocyte fusion and has a role in trophoblast fusion during placental formation.
CD46 also known as membrane cofactor protein (MCP) is a type I membrane protein with a molecular weight around 50-70 kDa due to glycosylation variability. This protein serves as a regulatory component in the complement system which protects host cells from damage by complement components. CD46 is widely expressed on almost all nucleated human cells. It plays a role in inactivating C3b and C4b components of the complement cascade preventing autologous complement-mediated damage. The gene coding for CD46 is located on chromosome 1q32 a region known for encoding several complement regulatory proteins.
CD46 integrates into various signaling pathways including the complement and T cell receptor signaling pathways. In the complement pathway CD46 interacts with C3b and factor I promoting cleavage and inactivation of C3b leading to pathway regulation. Involvement in the T cell receptor pathway hints that CD46 signaling protects against overactive immune responses by promoting a switch to a regulatory T cell phenotype which can modulate immunity and prevent autoimmunity.
CD46 has connections with autoimmune conditions and infectious diseases. For instance alterations in CD46 expression or function can contribute to atypical hemolytic uremic syndrome where dysregulation of the complement pathway occurs. Additionally certain pathogens like Neisseria gonorrhoeae utilize CD46 as a receptor for infection. CD46’s interaction with proteins such as C3b connects it directly to complement-related conditions highlighting its importance in maintaining immune system balance.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This WB data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: CD46 knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: K562 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108307 was shown to specifically react with when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-CD46 antibody [EPR4014] (AB108307)
Predicted band size: 44 kDa
This IHC data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).
ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human normal placenta tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human normal uterus tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human thyroid gland carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human colonic adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human breast carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ab108307 showing positive staining in human normal breast tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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