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Mouse Monoclonal CD47 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD47 aa 1-150.


Images

Key facts

Isotype

IgG2c

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human CD47 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q08722
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBProtein Array
Human
Tested
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Primary incubation for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Alternative names

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Mouse Monoclonal CD47 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD47 aa 1-150.

Alternative names

Key facts

Isotype

IgG2c

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human CD47 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q08722
Clone number

CD47/2937

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
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Purification notes

Purified from bioreactor concentrate.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.

Activity summary

CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.

Pathways

CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.

Associated diseases and disorders

CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260418), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260418)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

  • Western blot - Anti-CD47 antibody [CD47/2937] (ab260418), expandable thumbnail

    Western blot - Anti-CD47 antibody [CD47/2937] (ab260418)

    All lanes: Western blot - Anti-CD47 antibody [CD47/2937] (AB260418) at 2 µg/mL

    Lane 1: U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate

    Lane 2: THP-1 (human monocytic leukemia cell line) cell lysate

    Predicted band size: 35 kDa

  • Protein Array - Anti-CD47 antibody [CD47/2937] (ab260418), expandable thumbnail

    Protein Array - Anti-CD47 antibody [CD47/2937] (ab260418)

    Array of >19,000 full-length human proteins, probed with ab260418.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260418), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260418)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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