Anti-CD47 antibody [CD47/2937] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).

• WB

Supplier Data

Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).

All lanes:

Western blot - Anti-CD47 antibody [CD47/2937] (<a href='/en-us/products/primary-antibodies/cd47-antibody-cd47-2937-ab260418'>ab260418</a>) at 2 µg/mL

Lane 1:

U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Lane 2:

THP-1 (human monocytic leukemia cell line) cell lysate

Predicted band size: 35 kDa

false

• Protein Array

Supplier Data

Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)

Array of >19,000 full-length human proteins, probed with ab260418.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).