Mouse Monoclonal CD47 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD47 aa 1-150.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129357.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)")
![Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--western-blot-img24956.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)")
![Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--protein-array-img137442.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119037.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (AB260480)")
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD47 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q08722
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).
Alternative names
CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP
Recommended products
Mouse Monoclonal CD47 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD47 aa 1-150.
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD47 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q08722
- Carrier free
- Yes
- Clone number
- CD47/2937
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab260480 is the carrier-free version of Anti-CD47 antibody [CD47/2937] ab260418.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.
- Biological function summary
CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.
- Pathways
CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.
- Associated diseases and disorders
CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129357.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using Anti-CD47 antibody [CD47/2937] ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (Anti-CD47 antibody [CD47/2937] ab260418).
![Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--western-blot-img24956.jpg "Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)")
Western blot - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
This data was developed using the same antibody clone in a different formulation containing BSA and azide (Anti-CD47 antibody [CD47/2937] ab260418).
All lanes: Western blot - Anti-CD47 antibody [CD47/2937] (Anti-CD47 antibody [CD47/2937] ab260418) at 2 µg/mL
Lane 1: U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2: THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 35 kDa
![Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--protein-array-img137442.jpg "Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)")
Protein Array - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
Array of >19,000 full-length human proteins, probed with Anti-CD47 antibody [CD47/2937] ab260418.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (Anti-CD47 antibody [CD47/2937] ab260418).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-cd47-2937-bsa-and-azide-free-ab260480--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119037.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using Anti-CD47 antibody [CD47/2937] ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (Anti-CD47 antibody [CD47/2937] ab260418).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com