Rabbit Recombinant Monoclonal CD47 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (AB256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--flow-cytometry-img18309.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (AB256495)")
![Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (AB256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--flow-cytometry-img29577.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (AB256495)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (AB256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--immunocytochemistry-immunofluorescence-img407869.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (AB256495)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (AB256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--immunocytochemistry-immunofluorescence-img407868.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (AB256495)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).
Alternative names
MER6, CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP
Recommended products
Rabbit Recombinant Monoclonal CD47 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23002-67
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--flow-cytometry-img18309.jpg "Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495)")
Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495)
Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma, Left) / U-937 (Human monocyte histiocytic lymphoma, Right) cells labelling CD47 with ab256495 at 1/500 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody.
Low expression control: HepG2 (PMID: 25721088, 30415063).
Gated on viable cells.
![Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--flow-cytometry-img29577.jpg "Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495)")
Flow Cytometry - Anti-CD47 antibody [EPR23002-67] (ab256495)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD47 with ab256495 at 1/500 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--immunocytochemistry-immunofluorescence-img407869.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495)")
Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495)
Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) cells labelling CD47 with ab256495 at 1:100 (5.62 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 (2.5μg/ml) dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as the secondary antibody at 1:1000 (2 ug/ml) dilution. DAPI (blue) was used as nuclear counterstain. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as the secondary antibody only control.
![Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495), expandable thumbnail](https://content.abcam.com/products/images/cd47-antibody-epr23002-67-ab256495--immunocytochemistry-immunofluorescence-img407868.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495)")
Immunocytochemistry/ Immunofluorescence - Anti-CD47 antibody [EPR23002-67] (ab256495)
Immunocytochemistry analysis of CD47 KO HEK293T (Human CD47 knockout HEK-293T cell line ab266324) cells labelling CD47 with ab256495 at 1:100 (5.62 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 (2.5μg/ml) dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as the secondary antibody at 1:1000 (2 ug/ml) dilution. DAPI (blue) was used as nuclear counterstain. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as the secondary antibody only control.
Confocal image showing membranous staining in Parental HEK293 cell line, no staining in CD47 KO HEK293T cell line.
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