Anti-CD48 antibody [EPR26531-77]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (AB302708)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD48 with ab302708 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in Raji cell lineNegative control : HeLa (PMID : 9041467) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (AB302708)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD48 with ab302708 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (AB302708)

Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD48 with ab302708 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : HeLa (PMID : 9041467).

• WB

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Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID : 24626474). Negative controls : K-562, HeLa (PMID : 26878112, 9041467) Samples are non-boiled as boiling may cause protein aggregates. Exposure time : Lane 2 : 70 seconds Lane 1, 3-5 : 26 seconds

All lanes:

Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution

Lane 1:

Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate 20 µg

Lane 2:

Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate 20 µg

Lane 3:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate 20 µg

Lane 4:

K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 20 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 10 seconds

All lanes:

Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution

Lane 1:

Human lymph node tissue lysate 20 µg

Lane 2:

Human tonsil tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 10s