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Rabbit Recombinant Monoclonal CD48 antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (AB302708), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (AB302708), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (AB302708), expandable thumbnail
  • Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708), expandable thumbnail
  • Western blot - Anti-CD48 antibody [EPR26531-77] (AB302708), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-PWB
Human
Not recommended
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Target data

Function

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817).

Alternative names

BCM1, BLAST1, CD48, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, SLAMF2

Recommended products

Rabbit Recombinant Monoclonal CD48 antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26531-77

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD48 also known as SLAMF2 or B-lymphocyte activation marker BLAST-1 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass approximately 45-70 kDa depending on glycosylation. It is expressed on the surface of various immune cells like T cells B cells and natural killer (NK) cells. CD48 does not possess an intracellular signaling domain but engages in extracellular interactions with other proteins affecting immune cell activities.

Biological function summary

CD48 serves as a co-stimulatory molecule involved in immune response modulation. It interacts with 2B4 (CD244) a member of the signaling lymphocyte activation molecule (SLAM) family to form a receptor-ligand complex. This interaction can result in enhanced T cell and NK cell responses. CD48's presence on activated hematopoietic and non-hematopoietic cells highlights its role in cell-to-cell communication.

Pathways

CD48 participates in SLAM family receptor signaling and natural killer cell-mediated cytotoxicity. In the SLAM pathway CD48 enhances communication and regulation between T cells and NK cells. This communication also relates to the roles of CD244 linking it to downstream signaling pathways that influence immune cell function. These pathways illustrate the critical network CD48 is part of which impacts various immune responses.

Associated diseases and disorders

CD48 is involved in autoimmune conditions like rheumatoid arthritis and immunological cancers such as lymphoma. In rheumatoid arthritis CD48 along with CD244 modulates immune responses which can exacerbate inflammation. In lymphoma abnormal CD48 expression can contribute to tumor progression and immune evasion indicating its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (ab302708), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (ab302708)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD48 with ab302708 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (ab302708), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD48 antibody [EPR26531-77] (ab302708)

    Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD48 with ab302708 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control: HeLa (PMID: 9041467).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (ab302708), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD48 antibody [EPR26531-77] (ab302708)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD48 with ab302708 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in Raji cell lineNegative control: HeLa (PMID: 9041467? is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708), expandable thumbnail

    Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution

    Lane 1: Human lymph node tissue lysate 20 µg

    Lane 2: Human tonsil tissue lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45 kDa

    Exposure time: 10s

  • Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708), expandable thumbnail

    Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The molecular weight observed is consistent with what has been described in the literature (PMID: 24626474).

    Negative controls: K-562, HeLa (PMID: 26878112, 9041467)

    Samples are non-boiled as boiling may cause protein aggregates.
    Exposure time: Lane 2: 70 seconds
    Lane 1, 3-5: 26 seconds

    All lanes: Western blot - Anti-CD48 antibody [EPR26531-77] (ab302708) at 1/1000 dilution

    Lane 1: Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate 20 µg

    Lane 2: Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate 20 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate 20 µg

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 20 µg

    Lane 5: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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