Anti-CD48 antibody [EPR29721-7]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD48 antibody [EPR29721-7] (AB325192)

Flow cytometry staining of C57 BL/6 mouse splenocytes was performed using ab325192 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 minutes on ice in 1× PBS containing 10 µg/ml anti-CD16/CD32 and 10% normal goat serum to block Fc receptors and non-specific protein-protein interactions. They were then treated with either ab325192 or the isotype control antibody (1×10⁶ cells in 100 µl at 0.2 µg/ml, dilution 1 : 2500) for 30 minutes on ice. CD4 staining was performed simultaneously.

The secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488, ab150077), preadsorbed, was used at a 1 : 4000 dilution and incubated for 30 minutes on ice.

More than 30, 000 events were acquired using a 50 mW blue laser (488 nm) and a 525/40 bandpass filter. Events were gated on lymphocytes.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD48 antibody [EPR29721-7] (AB325192)

Flow cytometry overlay histogram shows positive A20 cells on the left and negative NIH3T3 cells on the right, stained with ab325192 (red line). Cells were incubated in 1× PBS containing 10 µg/ml anti-CD16/CD32 and 10% normal goat serum to block Fc receptors and non-specific protein-protein interactions, followed by staining with ab325192 (1×10⁶ cells in 100 µl at 0.2 µg/ml, dilution 1 : 2500) for 30 minutes on ice.

The secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488, ab150077), preadsorbed, was used at a 1 : 4000 dilution and incubated for 30 minutes on ice.

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control, used at the same concentration and conditions as the primary antibody. An unlabelled sample (blue line) was also included as a control.

More than 5, 000 events were acquired using a 50 mW blue laser (488 nm) and a 525/40 bandpass filter.