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AB248658

Anti-CD48 antibody [EPR4108] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD48 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD48, BCM1, BLAST1, CD48 antigen, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1, SLAMF2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)

This data was developed using ab134049, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD48 with Purified ab134049 at 1 : 5000 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)

This data was developed using ab134049, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD48 with Purified ab134049 at 1 : 5000 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Immunoprecipitation - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)
  • IP

Lab

Immunoprecipitation - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)

This data was developed using ab134049, the same antibody clone in a different buffer formulation.

CD48 was immunoprecipitated from 0.35 mg Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg with ab134049 at 1/100 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg

Lane 2 : ab134049 IP in Raji whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab134049 in Raji whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD48 antibody [EPR4108] (<a href='/en-us/products/primary-antibodies/cd48-antibody-epr4108-ab134049'>ab134049</a>)

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Western blot - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)
  • WB

Unknown

Western blot - Anti-CD48 antibody [EPR4108] - BSA and Azide free (AB248658)

This data was developed using ab134049, the same antibody clone in a different buffer formulation.

CD48 exists as highly glycosylated protein on the cell surface. The molecule weight observed is consistent with what has been described in the literature (PMID : 24626474, 1383383, 10429668)

All lanes:

Western blot - Anti-CD48 antibody [EPR4108] (<a href='/en-us/products/primary-antibodies/cd48-antibody-epr4108-ab134049'>ab134049</a>) at 1/5000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

NAMALWA (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 40-47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4108

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248658 is the carrier-free version of ab134049.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD48 also known as SLAMF2 or B-lymphocyte activation marker BLAST-1 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass approximately 45-70 kDa depending on glycosylation. It is expressed on the surface of various immune cells like T cells B cells and natural killer (NK) cells. CD48 does not possess an intracellular signaling domain but engages in extracellular interactions with other proteins affecting immune cell activities.
Biological function summary

CD48 serves as a co-stimulatory molecule involved in immune response modulation. It interacts with 2B4 (CD244) a member of the signaling lymphocyte activation molecule (SLAM) family to form a receptor-ligand complex. This interaction can result in enhanced T cell and NK cell responses. CD48's presence on activated hematopoietic and non-hematopoietic cells highlights its role in cell-to-cell communication.

Pathways

CD48 participates in SLAM family receptor signaling and natural killer cell-mediated cytotoxicity. In the SLAM pathway CD48 enhances communication and regulation between T cells and NK cells. This communication also relates to the roles of CD244 linking it to downstream signaling pathways that influence immune cell function. These pathways illustrate the critical network CD48 is part of which impacts various immune responses.

CD48 is involved in autoimmune conditions like rheumatoid arthritis and immunological cancers such as lymphoma. In rheumatoid arthritis CD48 along with CD244 modulates immune responses which can exacerbate inflammation. In lymphoma abnormal CD48 expression can contribute to tumor progression and immune evasion indicating its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed : 12007789, PubMed : 19494291, PubMed : 27249817, PubMed : 9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed : 19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed : 12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed : 27249817).
See full target information CD48
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
View all publications
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Product promise

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