Mouse Monoclonal CD5 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD5 aa 250-400.
Images
![Protein Array - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--protein-array-img141793.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102959.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940)")
![Western blot - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--western-blot-img23099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (AB237940)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD5 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link P06127
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
1 product for Alternative Version
Target data
Function
Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044).
Alternative names
CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1
Recommended products
Mouse Monoclonal CD5 antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD5 aa 250-400.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD5 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link P06127
- Carrier free
- Yes
- Clone number
- CD5/2416
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.
- Biological function summary
CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.
- Pathways
CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.
- Associated diseases and disorders
CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Protein Array - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--protein-array-img141793.jpg "Protein Array - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)")
Protein Array - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)
This data was produced with Anti-CD5 antibody [CD5/2416] ab238086, the same antibody in a different formulation with BSA and Azide.
Anti-CD5 antibody [CD5/2416] ab238086 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102959.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD5 using ab237940 at 2 μg/ml in immunohistochemical analysis.
![Western blot - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940), expandable thumbnail](https://content.abcam.com/products/images/cd5-antibody-cd5-2416-bsa-and-azide-free-ab237940--western-blot-img23099.jpg "Western blot - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)")
Western blot - Anti-CD5 antibody [CD5/2416] - BSA and Azide free (ab237940)
All lanes: Western blot - Anti-CD5 antibody [CD5/2416] (Anti-CD5 antibody [CD5/2416] ab238086) at 2 µg/mL
All lanes: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 54 kDa
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