AB327141

Anti-CD5 Antibody [CL12420]

Name: Anti-CD5 Antibody [CL12420] (ab327141) | Abcam
Brand: Abcam Limited
SKU: AB327141
Price: 540 USD
Availability: InStock

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Mouse Monoclonal CD5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CD5.

View Alternative Names

CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

Immunohistochemical staining of human chronic lymphocytic leukemia using ab327141 at 1 : 1000–1 : 2500 dilution, showing strong membranous positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

Immunohistochemical staining of human lymph node using ab327141 at 1 : 1000–1 : 2500 dilution, showing strong membranous positivity in germinal and non-germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

Immunohistochemical staining of human small intestine using ab327141 at 1 : 1000–1 : 2500 dilution, showing strong membranous positivity in lymphoid cells.

Immunocytochemistry/ Immunofluorescence - Anti-CD5 Antibody [CL12420] (AB327141)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD5 Antibody [CL12420] (AB327141)

Immunofluorescence staining of A-431 cells using ab327141 at 2–10 µg/ml, showing localization in plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

Immunohistochemistry analysis in human lymph node and skeletal muscle tissues using ab327141 at 1 : 1000–1 : 2500 dilution. Corresponding CD5 RNA-seq data are presented for the same tissues.

Supplier Data

Western blot - Anti-CD5 Antibody [CL12420] (AB327141)

Western blot analysis in human cell line MOLT-4 using ab327141 at 1 µg/ml.

All lanes:

Western blot - Anti-CD5 Antibody [CL12420] (ab327141)

All lanes:

Human cell line MOLT-4

Predicted band size: 54.58 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 Antibody [CL12420] (AB327141)

Immunohistochemical staining of human skeletal muscle using ab327141 at 1 : 1000–1 : 2500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL12420

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CD5.

P06127

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed : 1384049, PubMed : 1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed : 23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed : 27499044).

See full target information CD5

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Product promise

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