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Rabbit Recombinant Monoclonal CD5 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424), expandable thumbnail
  • Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424), expandable thumbnail
  • Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Tested
Tested
Not recommended
Not recommended
Expected

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance (PubMed:35720357). Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis (PubMed:24950378, PubMed:9064341, PubMed:9723705). Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:11157848). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (By similarity). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (By similarity).

Alternative names

Ly-1, Cd5, Ly-1, T-cell surface glycoprotein CD5, Lymphocyte antigen 1, Ly-1, Lyt-1

Recommended products

Rabbit Recombinant Monoclonal CD5 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR26533-75

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.

Biological function summary

CD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.

Pathways

CD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.

Associated diseases and disorders

CD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: A20 (PMID:19520434).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-CD5 antibody [EPR26533-75] (Anti-CD5 antibody [EPR26533-75] ab300423) at 1/1000 dilution

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

    Lane 2: A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 67 kDa

    Exposure time: 37s

  • Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.
    Negative control: Skeleal muscle(HPA)

    Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-CD5 antibody [EPR26533-75] (Anti-CD5 antibody [EPR26533-75] ab300423) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate

    Lane 2: Mouse skeletal muscle tissue lysate

    Lane 3: Rat spleen tissue lysate

    Lane 4: Rat skeletal muscle tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 67 kDa

    Exposure time: 147s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD5 with Anti-CD5 antibody [EPR26533-75] ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membraneous staining on mouse spleen.The section was incubated with Anti-CD5 antibody [EPR26533-75] ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD5 with Anti-CD5 antibody [EPR26533-75] ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membraneous staining on rat spleen.The section was incubated with Anti-CD5 antibody [EPR26533-75] ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.
    CD5 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 ug with Anti-CD5 antibody [EPR26533-75] ab300423 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD5 antibody [EPR26533-75] ab300423 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 ug
    Lane 2: abAB300423 IP in EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300424 in EL4 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (Anti-CD5 antibody [EPR26533-75] ab300423) at 1/1000 dilution

    Lanes 1 - 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

    CD5 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 ug with Anti-CD5 antibody [EPR26533-75] ab300423 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD5 antibody [EPR26533-75] ab300423 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 ug
    Lane 2: abAB300423 IP in EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300424 in EL4 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (ab300424)

    This data was developed using Anti-CD5 antibody [EPR26533-75] ab300423, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling CD5 with Anti-CD5 antibody [EPR26533-75] ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on mouse skeletal muscle.The section was incubated with Anti-CD5 antibody [EPR26533-75] ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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