Anti-CD5 antibody [EPR26533-75] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling CD5 with ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse skeletal muscle.The section was incubated with ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD5 with ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membraneous staining on rat spleen.The section was incubated with ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD5 with ab300423 at 1/5000 (0.104 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membraneous staining on mouse spleen.The section was incubated with ab300423 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation. CD5 was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate 10 ug with ab300423 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300423 at 1/1000 dilution. Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD5 antibody [EPR26533-75] (<a href='/en-us/products/primary-antibodies/cd5-antibody-epr26533-75-ab300423'>ab300423</a>) at 1/1000 dilution

Lanes 1 - 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300424 in EL4 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation. Negative control : Skeleal muscle(HPA) Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD5 antibody [EPR26533-75] (<a href='/en-us/products/primary-antibodies/cd5-antibody-epr26533-75-ab300423'>ab300423</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate

Lane 2:

Mouse skeletal muscle tissue lysate

Lane 3:

Rat spleen tissue lysate

Lane 4:

Rat skeletal muscle tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 67 kDa

false

Exposure time: 147s

• WB

Supplier Data

Western blot - Anti-CD5 antibody [EPR26533-75] (BSA and Azide free) (AB300424)

This data was developed using ab300423, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : A20 (PMID : 19520434).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-CD5 antibody [EPR26533-75] (<a href='/en-us/products/primary-antibodies/cd5-antibody-epr26533-75-ab300423'>ab300423</a>) at 1/1000 dilution

Lane 1:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

Lane 2:

A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 67 kDa

true

Exposure time: 37s