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AB275257

Anti-CD52 antibody [EPR23855-41] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD52 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD52, CDW52, HE5, CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5

1 Images
Flow Cytometry - Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (AB275257)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (AB275257)

This data was developed using ab259794, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD52 with ab259794 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control.

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG or ab259794. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

Gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23855-41

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab275257 is the carrier-free version of ab259794.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD52 also known as Campath-1 antigen is a glycoprotein that weighs approximately 12 kDa. It is mainly expressed on the surface of lymphocytes monocytes macrophages and some epithelial cells. This protein is also present on T and B cells making it a reliable marker for these immune cells. CD52's role is primarily in cell adhesion and signaling. The anti-CD52 antibody alemtuzumab targets this antigen specifically indicating its utility in clinical applications.
Biological function summary

CD52 plays an essential role in immune response regulation. It is not part of a larger protein complex but interacts dynamically with cell membranes to mediate immune cell activity. By acting as a co-stimulatory molecule for T cells CD52 influences lymphocyte activation and proliferation. This activity becomes significant in modulating immune responses showcasing its importance in immunological pathways.

Pathways

CD52 heavily influences T cell receptor signaling and cytokine production pathways. It interacts with proteins in these pathways to facilitate effective immune surveillance and response. CD52's engagement in these pathways highlights its collaboration with proteins such as CD3 and CD28 which assist in T cell activation and adaptive immunity. Such interactions highlight CD52's role in maintaining immune homeostasis.

CD52 has substantial relevance in autoimmune diseases and hematological cancers. Multiple sclerosis is one such autoimmune disorder where aberrant CD52 activity affects disease progression. Alemtuzumab an anti-CD52 monoclonal antibody is often used in treatment emphasizing CD52's clinical significance. In hematological malignancies like chronic lymphocytic leukemia CD52 facilitates abnormal lymphocyte survival and proliferation. CD52's interplay with other cell surface proteins such as CD19 further delineates its role in disease pathology and targeting strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
See full target information CD52
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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