AB125071

Anti-CD52 antibody [EPR3153(2)]

20ul selling size
RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CD52 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD52, Cdw52, Mb7, Cd52, CAMPATH-1 antigen, Lymphocyte differentiation antigen B7

1 Images

Unknown

Western blot - Anti-CD52 antibody [EPR3153(2)] (AB125071)

All lanes:

Western blot - Anti-CD52 antibody [EPR3153(2)] (ab125071) at 1/1000 dilution

Lane 1:

Mouse thymus lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 6 kDa

true

Carrier free

Anti-CD52 antibody [EPR3153(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3153(2)

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD52 also known as Campath-1 antigen is a glycoprotein that weighs approximately 12 kDa. It is mainly expressed on the surface of lymphocytes monocytes macrophages and some epithelial cells. This protein is also present on T and B cells making it a reliable marker for these immune cells. CD52's role is primarily in cell adhesion and signaling. The anti-CD52 antibody alemtuzumab targets this antigen specifically indicating its utility in clinical applications.

Biological function summary

CD52 plays an essential role in immune response regulation. It is not part of a larger protein complex but interacts dynamically with cell membranes to mediate immune cell activity. By acting as a co-stimulatory molecule for T cells CD52 influences lymphocyte activation and proliferation. This activity becomes significant in modulating immune responses showcasing its importance in immunological pathways.

Pathways

CD52 heavily influences T cell receptor signaling and cytokine production pathways. It interacts with proteins in these pathways to facilitate effective immune surveillance and response. CD52's engagement in these pathways highlights its collaboration with proteins such as CD3 and CD28 which assist in T cell activation and adaptive immunity. Such interactions highlight CD52's role in maintaining immune homeostasis.

CD52 has substantial relevance in autoimmune diseases and hematological cancers. Multiple sclerosis is one such autoimmune disorder where aberrant CD52 activity affects disease progression. Alemtuzumab an anti-CD52 monoclonal antibody is often used in treatment emphasizing CD52's clinical significance. In hematological malignancies like chronic lymphocytic leukemia CD52 facilitates abnormal lymphocyte survival and proliferation. CD52's interplay with other cell surface proteins such as CD19 further delineates its role in disease pathology and targeting strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

See full target information Cd52

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:31441 PubMed39733159

2024

Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation.

Applications

Unspecified application

Species

Unspecified reactive species

Wayne Hicks,Sirsendu Jana,Tigist Kassa,Richard Prince,Pedro Cabrales,Joel Friedman,Abdu I Alayash

Molecular medicine (Cambridge, Mass.) 29:145 PubMed37884883

2023

Leveraging a disulfidptosis-related signature to predict the prognosis and immunotherapy effectiveness of cutaneous melanoma based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Yanjun Wei,Lingjia Fan,Yuanliu Nie,Jianan Li,Renya Zeng,Jixian Li,Xiang Zhan,Lingli Lei,Zhichao Kang,Jiaxin Li,Wentao Zhang,Zhe Yang

Nature biotechnology 41:344-354 PubMed36302989

2022

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Dai,Li-Sheng Zhang,Hui-Lung Sun,Kinga Pajdzik,Lei Yang,Chang Ye,Cheng-Wei Ju,Shun Liu,Yuru Wang,Zhong Zheng,Linda Zhang,Bryan T Harada,Xiaoyang Dou,Iryna Irkliyenko,Xinran Feng,Wen Zhang,Tao Pan,Chuan He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD52 antibody [EPR3153(2)]

Western blot - Anti-CD52 antibody [EPR3153(2)] (AB125071)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise