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AB194860

Anti-CD52 antibody [YTH34.5] - Low endotoxin, Azide free

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(1 Publication)

Rat Monoclonal CD52 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD52 protein.

View Alternative Names

CD52, CDW52, HE5, CAMPATH-1 antigen, CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, Human epididymis-specific protein 5, He5

1 Images
Flow Cytometry - Anti-CD52 antibody [YTH34.5] - Low endotoxin, Azide free (AB194860)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD52 antibody [YTH34.5] - Low endotoxin, Azide free (AB194860)

Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD52 with ab194860 at 1/50 dilution.

  • 578 PE

    PE Anti-CD52 antibody [YTH34.5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

YTH34.5

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD52 protein. The exact immunogen used to generate this antibody is proprietary information.

P31358

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p>Use 10 μl of the suggested working dilution to label 1 x 106 cells in 100 μl.</p><p> </p><p> </p><p> </p><p> </p><p><a href='/en-us/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab194860 was prepared by affinity chromatography on Protein A from tissue culture supernatant.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD52 also known as Campath-1 antigen is a glycoprotein that weighs approximately 12 kDa. It is mainly expressed on the surface of lymphocytes monocytes macrophages and some epithelial cells. This protein is also present on T and B cells making it a reliable marker for these immune cells. CD52's role is primarily in cell adhesion and signaling. The anti-CD52 antibody alemtuzumab targets this antigen specifically indicating its utility in clinical applications.
Biological function summary

CD52 plays an essential role in immune response regulation. It is not part of a larger protein complex but interacts dynamically with cell membranes to mediate immune cell activity. By acting as a co-stimulatory molecule for T cells CD52 influences lymphocyte activation and proliferation. This activity becomes significant in modulating immune responses showcasing its importance in immunological pathways.

Pathways

CD52 heavily influences T cell receptor signaling and cytokine production pathways. It interacts with proteins in these pathways to facilitate effective immune surveillance and response. CD52's engagement in these pathways highlights its collaboration with proteins such as CD3 and CD28 which assist in T cell activation and adaptive immunity. Such interactions highlight CD52's role in maintaining immune homeostasis.

CD52 has substantial relevance in autoimmune diseases and hematological cancers. Multiple sclerosis is one such autoimmune disorder where aberrant CD52 activity affects disease progression. Alemtuzumab an anti-CD52 monoclonal antibody is often used in treatment emphasizing CD52's clinical significance. In hematological malignancies like chronic lymphocytic leukemia CD52 facilitates abnormal lymphocyte survival and proliferation. CD52's interplay with other cell surface proteins such as CD19 further delineates its role in disease pathology and targeting strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
See full target information CD52
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 17:e12825 PubMed30094915

2018

A molecular signature for delayed graft function.

Applications

Unspecified application

Species

Unspecified reactive species

Dagmara McGuinness,Suhaib Mohammed,Laura Monaghan,Paul A Wilson,David B Kingsmore,Oliver Shapter,Karen S Stevenson,Shana M Coley,Luke Devey,Robert B Kirkpatrick,Paul G Shiels
View all publications
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Product promise

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