AB134094

Anti-CD53 antibody [EPR4342(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CD53 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD53, MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25

4 Images

Flow Cytometry (Intracellular) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD53 with unpurified ab134094 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD53 with ab134094 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD53 antibody [EPR4342(2)] (AB134094)

All lanes:

Western blot - Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

PBMC cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Lab

Western blot - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Blocking and dilution buffer : 5% NFDM/TBST.

We are not sure how to define the extra band.

All lanes:

Western blot - Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/5000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate. lysate was treated with Deglycosylase at 1/20 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 20 kDa

false

Exposure time: 180s

Carrier free

Anti-CD53 antibody [EPR4342(2)] - BSA and Azide free
578 PE

PE Anti-CD53 antibody [EPR4342(2)]
660 APC

APC Anti-CD53 antibody [EPR4342(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD53 antibody [EPR4342(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD53 antibody [EPR4342(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD53 antibody [EPR4342(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD53 antibody [EPR4342(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD53 antibody [EPR4342(2)]
HRP

HRP Anti-CD53 antibody [EPR4342(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD53 antibody [EPR4342(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4342(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD53 also known as Tetraspanin-25 is a transmembrane glycoprotein with a molecular mass of approximately 35-40 kDa. It belongs to the tetraspanin family characterized by four transmembrane domains. CD53 primarily resides on the surface of leukocytes including B cells T cells natural killer cells and macrophages. This protein plays an important role in signal transduction processes by organizing proteins on the cell membrane and forming tetraspanin-enriched microdomains.

Biological function summary

CD53 plays a significant role in regulating immune responses cell development and migration. It does not work alone but as part of larger protein complexes that influence cell communication and inter-organizational responses. CD53 participates in organizing membrane proteins into functional complexes which impacts cellular processes such as adhesion signaling and cellular activation. Its interaction with other proteins is critical to maintain normal immune system functionality and responding appropriately to infections.

Pathways

CD53 is involved in the regulation of immune signaling and cellular communication networks. It is a component of the tetraspanin web which influences the integrin and immunoreceptor pathways two important signaling pathways in cellular communication. Proteins such as CD9 and CD81 also members of the tetraspanin family frequently interact with CD53 suggesting cooperative roles in modulating cellular adhesion and trafficking processes critical for immune response.

CD53 shows a connection to immune system dysfunctions including autoimmune conditions and certain types of cancer. Its altered expression can contribute to abnormal immune responses seen in diseases like systemic lupus erythematosus (SLE) and lymphoma. In these disorders CD53's interaction with CD19 and other B-cell-associated proteins plays a part in abnormal cell signaling leading to issues in cell proliferation or miscommunication within the immune system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed : 32974937, PubMed : 35767951). Plays a role in the activation of monocytes and B-cells (PubMed : 8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed : 28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity).

See full target information CD53

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:571 PubMed39112478

2024

Single-cell transcriptome profiles the heterogeneity of tumor cells and microenvironments for different pathological endometrial cancer and identifies specific sensitive drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Ren,Lingfang Wang,Yuyouye Wang,Jiaxuan Wang,Yuanpei Wang,Xiaole Song,Gong Zhang,Fangfang Nie,Shitong Lin

Nature communications 13:4121 PubMed35840578

2022

The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ian D Ferguson,Bonell Patiño-Escobar,Sami T Tuomivaara,Yu-Hsiu T Lin,Matthew A Nix,Kevin K Leung,Corynn Kasap,Emilio Ramos,Wilson Nieves Vasquez,Alexis Talbot,Martina Hale,Akul Naik,Audrey Kishishita,Priya Choudhry,Antonia Lopez-Girona,Weili Miao,Sandy W Wong,Jeffrey L Wolf,Thomas G Martin,Nina Shah,Scott Vandenberg,Sonam Prakash,Lenka Besse,Christoph Driessen,Avery D Posey,R Dyche Mullins,Justin Eyquem,James A Wells,Arun P Wiita

International journal of molecular sciences 22: PubMed33401449

2021

The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Montanaro,Manuel Scimeca,Lucia Anemona,Francesca Servadei,Erica Giacobbi,Rita Bonfiglio,Elena Bonanno,Nicoletta Urbano,Arnaldo Ippoliti,Giuseppe Santeusanio,Orazio Schillaci,Alessandro Mauriello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD53 antibody [EPR4342(2)]

Flow Cytometry (Intracellular) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Western blot - Anti-CD53 antibody [EPR4342(2)] (AB134094)

Western blot - Anti-CD53 antibody [EPR4342(2)] (AB134094)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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