AB180546

Anti-CD55 antibody [3D5]

Name: Anti-CD55 antibody [3D5] (ab180546) | Abcam
Brand: Abcam Limited
SKU: AB180546
Price: 495 USD
Availability: InStock

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(2 Publications)

Rat Monoclonal DAF1 antibody. Suitable for Flow Cyt, WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd55b protein.

View Alternative Names

CD55, Cd55a, Daf, Daf1, Cd55, DAF-GPI

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

3D5

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, WB

applications

Immunogen

Cell preparation containing Cd55b protein. The exact immunogen used to generate this antibody is proprietary information.

Q61476

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.

Biological function summary

CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.

Pathways

CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.

A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

See full target information Cd55

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 236:5012-5021 PubMed33400289

2021

Uromodulin aggravates renal tubulointerstitial injury through activation of the complement pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Fei Pei,Qiaoling Sun,Fei Shen,Xiangdong Yang,Zhao Hu,Maojing Liu

Journal of neuroinflammation 11:115 PubMed24952384

2014

Glimepiride reduces CD14 expression and cytokine secretion from macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Ingham,Alun Williams,Clive Bate

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD55 antibody [3D5]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise