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AB20145

Anti-CD55 antibody [67]

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(6 Publications)

Mouse Monoclonal DAF antibody. Suitable for IHC-Fr and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD55 protein.

View Alternative Names

CD55, CR, DAF, Complement decay-accelerating factor

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

67

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

Cell preparation containing CD55 protein. The exact immunogen used to generate this antibody is proprietary information.

P08174

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Fix with acetone.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.
Biological function summary

CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.

Pathways

CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.

A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed : 7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed : 28657829).. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).. (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
See full target information CD55
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 13:72 PubMed27056040

2016

Complement activation at the motor end-plates in amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nawal Bahia El Idrissi,Sanne Bosch,Valeria Ramaglia,Eleonora Aronica,Frank Baas,Dirk Troost

Cancer science 100:2411-8 PubMed19758394

2009

Engineered anti-CD20 antibodies with enhanced complement-activating capacity mediate potent anti-lymphoma activity.

Applications

Unspecified application

Species

Unspecified reactive species

Akito Natsume,Yukiko Shimizu-Yokoyama,Mitsuo Satoh,Kenya Shitara,Rinpei Niwa

Pathology international 59:382-9 PubMed19490468

2009

Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720.

Applications

IHC-Fr

Species

Human

Kazuo Kamada,Norimasa Arita,Takahito Tsubaki,Nobuo Takubo,Takahiro Fujino,Yoshiko Soga,Tatsuhiko Miyazaki,Haruyasu Yamamoto,Masato Nose

Molecular human reproduction 14:413-21 PubMed18524812

2008

Expression of immunomodulatory genes, their protein products and specific ligands/receptors during the window of implantation in the human endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Anahi Franchi,Jessica Zaret,Xiaomei Zhang,Silvina Bocca,Sergio Oehninger

Journal of cell science 115:963-72 PubMed11870215

2002

The involvement of lipid rafts in the regulation of integrin function.

Applications

Unspecified application

Species

Unspecified reactive species

Birgit Leitinger,Nancy Hogg

Clinical and experimental immunology 59:529-38 PubMed3886218

1985

Features of synovial membrane identified with monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

D G Palmer,Y Selvendran,C Allen,P A Revell,N Hogg
View all publications
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