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Rabbit Recombinant Monoclonal DAF1 antibody. Carrier free. Suitable for Dot, Flow Cyt and reacts with Transfected cell lysate - Mouse, Mouse samples.

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Images

Dot Blot - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068), expandable thumbnail
  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068), expandable thumbnail
  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068), expandable thumbnail
  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotFlow CytIHC-PWB
Mouse
Expected
Tested
Not recommended
Not recommended
Transfected cell lysate - Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Target data

Function

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

Alternative names

CD55, Cd55a, Daf, Daf1, Cd55, DAF-GPI

Rabbit Recombinant Monoclonal DAF1 antibody. Carrier free. Suitable for Dot, Flow Cyt and reacts with Transfected cell lysate - Mouse, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28962-549
Purification technique
Affinity purification Protein A
Specificity

This antibody does not cross-react with mouse DAF2

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab324068 is the carrier free version of Anti-CD55 antibody [EPR28962-549] ab324067.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068), expandable thumbnail

    Dot Blot - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068)

    CD55 Dot Blot staining using rabbit Anti-CD55 antibody

    This data was developed using ab323067, the same antibody clone in a different buffer formulation.

    Dot blot analysis of CD55 using Anti-CD55 antibody [EPR28962-549] ab324067 at 1:1000 (0.489 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane 1: 293T cells transfected with a mouse CD55 expression vector containing a myc-His-tag®, whole cell lysate

    Lane 2: 293T cells transfected with a mouse DAF2 expression vector containing a myc-His-tag®, whole cell lysate

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with mouse DAF2 .

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-CD55 antibody [EPR28962-549] (Anti-CD55 antibody [EPR28962-549] ab324067) at 1/1000 dilution

    Lane 1: 293T cells transfected with a mouse CD55 expression vector containing a myc-His-tag®, whole cell lysate

    Lane 2: 293T cells transfected with a mouse DAF2 expression vector containing a myc-His-tag®, whole cell lysate

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068), expandable thumbnail

    Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068)

    CD55 Flow Cytometry staining of Mouse splenocytes using rabbit Anti-CD55 antibody

    This data was developed using ab323067, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse splenocytes cells labelling CD55 with Anti-CD55 antibody [EPR28962-549] ab324067 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse splenocytes were co-stained with CD19 conjugated PE/Cy7 (Green) and CD3 conjugated Alexa Fluor®647 (Magenta).

    Gated on viable cells.

    PMID: 11683961

  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068), expandable thumbnail

    Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068)

    CD55 Flow Cytometry staining of Mouse splenocytes using rabbit Anti-CD55 antibody

    This data was developed using ab323067, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse splenocytes cells labelling CD55 with Anti-CD55 antibody [EPR28962-549] ab324067 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse splenocytes (Right) were co-stained with CD8 conjugated BV421 and CD4 conjugated Alexa Fluor®647.

    Gated on viable CD8-CD4+ (Upper left) and CD4-CD8+ (Lower left) single positive populations (PMID: 25367120).

  • Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068), expandable thumbnail

    Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (ab324068)

    CD55 Flow Cytometry staining of Mouse thymus cell using rabbit Anti-CD55 antibody

    This data was developed using ab323067, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse thymus cell cells labelling CD55 with Anti-CD55 antibody [EPR28962-549] ab324067 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse thymus cells were co-stained with CD8 conjugated BV421 and CD4 conjugated Alexa Fluor®647.

    Gated on viable CD8+CD4+ double positive population (PMID: 25367120).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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