Anti-CD55 antibody [EPR28962-549] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068)

This data was developed using ab323067, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse thymus cell cells labelling CD55 with ab324067 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse thymus cells were co-stained with CD8 conjugated BV421 and CD4 conjugated Alexa Fluor®647.
Gated on viable CD8+CD4+ double positive population (PMID : 25367120).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068)

This data was developed using ab323067, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling CD55 with ab324067 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse splenocytes were co-stained with CD19 conjugated PE/Cy7 (Green) and CD3 conjugated Alexa Fluor®647 (Magenta).
Gated on viable cells.
PMID : 11683961

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068)

This data was developed using ab323067, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling CD55 with ab324067 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse splenocytes (Right) were co-stained with CD8 conjugated BV421 and CD4 conjugated Alexa Fluor®647.
Gated on viable CD8-CD4+ (Upper left) and CD4-CD8+ (Lower left) single positive populations (PMID : 25367120).

• Dot

Supplier Data

Dot Blot - Anti-CD55 antibody [EPR28962-549] - BSA and Azide free (AB324068)

This data was developed using ab323067, the same antibody clone in a different buffer formulation.

Dot blot analysis of CD55 using ab324067 at 1 : 1000 (0.489 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : 293T cells transfected with a mouse CD55 expression vector containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T cells transfected with a mouse DAF2 expression vector containing a myc-His-tag®, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse DAF2 .

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-CD55 antibody [EPR28962-549] (<a href='/en-us/products/primary-antibodies/cd55-antibody-epr28962-549-ab324067'>ab324067</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse CD55 expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a mouse DAF2 expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s