AB230797

Anti-CD55 antibody [EPR6689] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal DAF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD55, CR, DAF, Complement decay-accelerating factor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue sections labeling CD55 with purified ab133684 at 1/4000 dilution (0.04 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133684)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

This IHC data was generated using the same anti-CD55 antibody clone, EPR6689, in a different buffer formulation (cat# ab133684).

Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD55 with ab133684 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

This IHC data was generated using the same anti-CD55 antibody clone, EPR6689, in a different buffer formulation (cat# ab133684).

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with ab133684 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-CD55 antibody [EPR6689]
Biotin

Biotin Anti-CD55 antibody [EPR6689]
HRP

HRP Anti-CD55 antibody [EPR6689]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6689

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab230797 is the carrier-free version of ab133684.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.

Biological function summary

CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.

Pathways

CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.

A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed : 7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed : 28657829).. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).. (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.

See full target information CD55

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 42:266-282.e8 PubMed38278150

2024

Inosine induces stemness features in CAR-T cells and enhances potency.

Applications

Unspecified application

Species

Unspecified reactive species

Dorota D Klysz,Carley Fowler,Meena Malipatlolla,Lucille Stuani,Katherine A Freitas,Yiyun Chen,Stefanie Meier,Bence Daniel,Katalin Sandor,Peng Xu,Jing Huang,Louai Labanieh,Vimal Keerthi,Amaury Leruste,Malek Bashti,Janette Mata-Alcazar,Nikolaos Gkitsas,Justin A Guerrero,Chris Fisher,Sunny Patel,Kyle Asano,Shabnum Patel,Kara L Davis,Ansuman T Satpathy,Steven A Feldman,Elena Sotillo,Crystal L Mackall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD55 antibody [EPR6689] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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