Rabbit Recombinant Monoclonal DAF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107372.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105218.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100282.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)")
![OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--oi-rd-scanning-img97714.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (AB230797)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
1 product for Alternative Version
Target data
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829). (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
Alternative names
CD55, CR, DAF, Complement decay-accelerating factor
Recommended products
Rabbit Recombinant Monoclonal DAF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6689
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.25 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab230797 is the carrier-free version of Anti-CD55 antibody [EPR6689] ab133684.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.
- Biological function summary
CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.
- Pathways
CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.
- Associated diseases and disorders
A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107372.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue sections labeling CD55 with purified Anti-CD55 antibody [EPR6689] ab133684 at 1/4000 dilution (0.04 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD55 antibody [EPR6689] ab133684)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105218.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)
This IHC data was generated using the same anti-CD55 antibody clone, EPR6689, in a different buffer formulation (cat# Anti-CD55 antibody [EPR6689] ab133684).
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with Anti-CD55 antibody [EPR6689] ab133684 at 1/100 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100282.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)
This IHC data was generated using the same anti-CD55 antibody clone, EPR6689, in a different buffer formulation (cat# Anti-CD55 antibody [EPR6689] ab133684).
Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD55 with Anti-CD55 antibody [EPR6689] ab133684 at 1/100 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797), expandable thumbnail](https://content.abcam.com/products/images/cd55-antibody-epr6689-bsa-and-azide-free-ab230797--oi-rd-scanning-img97714.jpg "OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)")
OI-RD Scanning - Anti-CD55 antibody [EPR6689] - BSA and Azide free (ab230797)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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