Mouse Monoclonal CD57 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD57 protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (AB212403), expandable thumbnail](https://content.abcam.com/products/images/cd57-antibody-hnk-1-or-leu-7-bsa-and-azide-free-ab212403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108237.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (AB212403)")
Publications
- Frontiers in immunology 11:14662020A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Guo et. al.PubMed 32765508
Key facts
- Isotype
IgM
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing CD57 protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Rat | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-4 µg/mL | Notes for 30 minutes at RT Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes for 30 minutes at RT Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
12 products for Alternative Product
1 products for Alternative Version
Target data
Alternative names
HKN1, LEU7
Recommended products
Mouse Monoclonal CD57 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD57 protein.
Alternative names
HKN1, LEU7
Key facts
- Isotype
IgM
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing CD57 protein. The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
HNK-1 or Leu-7
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
ab212403 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
CD57 also known as HNK-1 is a glycoprotein with a molecular weight of approximately 110 to 150 kDa. It is a marker expressed on the surface of natural killer (NK) cells T lymphocytes and some subsets of CD8+ T cells. Besides you can find this marker in neural tissue. As a surface marker CD57 is used in human immunohistochemistry (IHC) studies to identify cell populations in immune responses and has significant utility in the characterization of lymphocyte subsets.
- Biological function summary
CD57 plays a role in immune regulation and cell-mediated cytotoxicity. CD57+ cells which are often mature or post-activation states in NK cells and T lymphocytes are less proliferative but have enhanced cytotoxic abilities. These cells contribute to the immune surveillance against viral infections and tumor cells. CD57 does not belong to any well-known protein complexes but can cooperate with other surface markers to modulate immune functions.
- Pathways
CD57+ cell populations participate in the immune system pathways related to cell-mediated immunity and cytotoxic response. The presence of CD57 is often associated with the downregulation of proliferative pathways and the enhancement of cytotoxic pathways. It shows functional overlap with proteins such as CD16 and NKG2D which also play roles in mediating the cytotoxic activity of NK cells.
- Associated diseases and disorders
CD57 is connected to chronic infections and certain autoimmune conditions. In chronic viral infections like HIV CD57+ T cells show a state of replicative senescence where their persistent expression links to disease progression. In autoimmune disorders like rheumatoid arthritis CD57+ T cells are found in the inflamed tissues. This protein works alongside CD8 in conditions of immune dysfunction providing insights into immune status and disease activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (ab212403), expandable thumbnail](https://content.abcam.com/products/images/cd57-antibody-hnk-1-or-leu-7-bsa-and-azide-free-ab212403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108237.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (ab212403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (ab212403)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD57 with ab212403 at 4μg/ml.
Downloads
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