AB212403

Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free

5

(1 Review)

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(2 Publications)

Mouse Monoclonal CD57 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD57 protein.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (AB212403)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (AB212403)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD57 with ab212403 at 4μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HNK-1 or Leu-7

Isotype

IgM

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p>for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2 - 1/4", "IHCP-species-notes": "<p>for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab212403 is purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD57 also known as HNK-1 is a glycoprotein with a molecular weight of approximately 110 to 150 kDa. It is a marker expressed on the surface of natural killer (NK) cells T lymphocytes and some subsets of CD8+ T cells. Besides you can find this marker in neural tissue. As a surface marker CD57 is used in human immunohistochemistry (IHC) studies to identify cell populations in immune responses and has significant utility in the characterization of lymphocyte subsets.

Biological function summary

CD57 plays a role in immune regulation and cell-mediated cytotoxicity. CD57+ cells which are often mature or post-activation states in NK cells and T lymphocytes are less proliferative but have enhanced cytotoxic abilities. These cells contribute to the immune surveillance against viral infections and tumor cells. CD57 does not belong to any well-known protein complexes but can cooperate with other surface markers to modulate immune functions.

Pathways

CD57+ cell populations participate in the immune system pathways related to cell-mediated immunity and cytotoxic response. The presence of CD57 is often associated with the downregulation of proliferative pathways and the enhancement of cytotoxic pathways. It shows functional overlap with proteins such as CD16 and NKG2D which also play roles in mediating the cytotoxic activity of NK cells.

CD57 is connected to chronic infections and certain autoimmune conditions. In chronic viral infections like HIV CD57+ T cells show a state of replicative senescence where their persistent expression links to disease progression. In autoimmune disorders like rheumatoid arthritis CD57+ T cells are found in the inflamed tissues. This protein works alongside CD8 in conditions of immune dysfunction providing insights into immune status and disease activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information CD57

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0262062 PubMed35536781

2022

Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies.

Applications

Unspecified application

Species

Unspecified reactive species

David Choy Buentello,Lena Sophie Koch,Grissel Trujillo-de Santiago,Mario Moisés Alvarez,Kerensa Broersen

Frontiers in immunology 11:1466 PubMed32765508

2020

A 34-Marker Panel for Imaging Mass Cytometric Analysis of Human Snap-Frozen Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Guo,Vincent van Unen,Marieke E Ijsselsteijn,Laura F Ouboter,Andrea E van der Meulen,Susana M Chuva de Sousa Lopes,Noel F C C de Miranda,Frits Koning,Na Li

View all publications

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