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AB196648

Anti-CD58 antibody [EP15041]

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(4 Publications)

Rabbit Recombinant Monoclonal CD58 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD58, LFA3, Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3

5 Images
Western blot - Anti-CD58 antibody [EP15041] (AB196648)
  • WB

Supplier Data

Western blot - Anti-CD58 antibody [EP15041] (AB196648)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/5000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 65-70 kDa

false

Western blot - Anti-CD58 antibody [EP15041] (AB196648)
  • WB

Supplier Data

Western blot - Anti-CD58 antibody [EP15041] (AB196648)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

Lane 1:

TF-1 (Human bone marrow erythroleukemia cells) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 65-70 kDa

false

Western blot - Anti-CD58 antibody [EP15041] (AB196648)
  • WB

Supplier Data

Western blot - Anti-CD58 antibody [EP15041] (AB196648)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed band size : 65-70 (27, 28) kDa.

All lanes:

Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/2000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma cell line) control at 10 µg

Lane 2:

SW480 (Human colorectal adenocarcinoma cell line) de-glycosylation at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

Western blot - Anti-CD58 antibody [EP15041] (AB196648)
  • WB

Lab

Western blot - Anti-CD58 antibody [EP15041] (AB196648)

Lanes 1 - 4 : Merged signal (red and green). Green - ab196648 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab196648 was shown to specifically react with CD58 in wild-type HAP1 cells as signal was lost in CD58 knockout cells. Wild-type and CD58 knockout samples were subjected to SDS-PAGE. ab196648 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 40 µg

Lane 2:

CD58 knockout HAP1 whole cell lysate at 40 µg

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Western blot - Anti-CD58 antibody [EP15041] (AB196648)
  • WB

Lab

Western blot - Anti-CD58 antibody [EP15041] (AB196648)

Lanes 1-4 : Merged signal (red and green). Green - ab196648 observed at 43 kDa. Red - loading control ab8245 observed at 37 kDa.

ab196648 Anti-CD58 antibody [EP15041] was shown to specifically react with CD58 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265947 (knockout cell lysate ab257880) was used. Wild-type and CD58 knockout samples were subjected to SDS-PAGE. ab196648 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CD58 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CD58 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cd58-knockout-hela-cell-line-ab265947'>ab265947</a>)

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

  • Carrier free

    Anti-CD58 antibody [EP15041] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP15041

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD58 also known as lymphocyte function-associated antigen 3 (LFA-3) is a glycoprotein with a molecular mass of approximately 60 kDa. It is present on the surface of various cell types including antigen-presenting cells epithelial cells and some other leukocytes. This protein serves as a ligand for CD2 which is expressed on T cells and natural killer cells. Through its interaction with CD2 CD58 mediates cell adhesion and plays a role in immunological synapse formation which is essential for effective immune responses.
Biological function summary

Lymphocyte function-associated antigen 3 acts as a significant participant in the immune system. It plays a role in T cell activation and facilitates interactions between T cells and antigen-presenting cells. CD58 forms part of a complex network of cell surface proteins that enhances immune surveillance and response. Its role highlights its importance in adaptive immunity by promoting cell-to-cell communication and activation.

Pathways

CD58 participates in the cell adhesion pathways. It interacts closely with CD2 which is involved in the activation and signaling of T cells. Through this interaction CD58 plays roles in pathways like T cell receptor signaling and immune response. It enhances cell-cell adhesion helping T cells maintain their position during immune surveillance and activation.

CD58 relates to conditions such as autoimmune diseases and certain cancers. In autoimmune disorders aberrant expression or function of CD58 may contribute to inappropriate immune activation leading to tissue damage. In cancer CD58 alterations can affect tumor immune evasion influencing how the immune system recognizes and attacks cancer cells. In multiple sclerosis an autoimmune disease the interaction between CD58 and CD2 has been studied providing insights into its role in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
See full target information CD58
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 41:1817-1828.e9 PubMed37683639

2023

CMTM6 shapes antitumor T cell response through modulating protein expression of CD58 and PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Beiping Miao,Zhaoqing Hu,Riccardo Mezzadra,Lotte Hoeijmakers,Astrid Fauster,Shangce Du,Zhi Yang,Melanie Sator-Schmitt,Helena Engel,Xueshen Li,Caroline Broderick,Guangzhi Jin,Raquel Gomez-Eerland,Lisette Rozeman,Xin Lei,Hitoshi Matsuo,Chen Yang,Ingrid Hofland,Dennis Peters,Annegien Broeks,Elke Laport,Annika Fitz,Xiyue Zhao,Mohamed A A Mahmoud,Xiujian Ma,Sandrine Sander,Hai-Kun Liu,Guoliang Cui,Yu Gan,Wei Wu,Yanling Xiao,Albert J R Heck,Wenxian Guan,Scott W Lowe,Hugo M Horlings,Cun Wang,Thijn R Brummelkamp,Christian U Blank,Ton N M Schumacher,Chong Sun

Cancer cell 41:1207-1221.e12 PubMed37327789

2023

The CD58-CD2 axis is co-regulated with PD-L1 via CMTM6 and shapes anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Ho,Johannes C Melms,Meri Rogava,Chris J Frangieh,Joanna Poźniak,Shivem B Shah,Zachary Walsh,Oleksandr Kyrysyuk,Amit Dipak Amin,Lindsay Caprio,Benjamin T Fullerton,Rajesh Kumar Soni,Casey R Ager,Jana Biermann,Yiping Wang,Mohsen Khosravi-Maharlooei,Giorgia Zanetti,Michael Mu,Hijab Fatima,Emily K Moore,Neil Vasan,Samuel F Bakhoum,Steven L Reiner,Chantale Bernatchez,Megan Sykes,Emily M Mace,Kai W Wucherpfennig,Dirk Schadendorf,Oliver Bechter,Parin Shah,Gary K Schwartz,Jean-Christophe Marine,Benjamin Izar

Science advances 8:eadd6403 PubMed36516256

2022

PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Li,Takaya Moriyama,Satoshi Yoshimura,Xujie Zhao,Zhenhua Li,Xu Yang,Elisabeth Paietta,Mark R Litzow,Marina Konopleva,Jiyang Yu,Hiroto Inaba,Raul C Ribeiro,Ching-Hon Pui,Jun J Yang

PloS one 15:e0231655 PubMed32325480

2020

Immune complex disease in a chronic monkey study with a humanised, therapeutic antibody against CCL20 is associated with complement-containing drug aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Susan B Laffan,Andrew S Thomson,Shing Mai,Cindy Fishman,Takahito Kambara,Kiran Nistala,James T Raymond,Shugui Chen,Thulasi Ramani,Laura Pageon,Rodd Polsky,Mark Watkins,Gemma Ottolangui,John R White,Curtis Maier,Michael Herdman,Gerben Bouma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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