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Rabbit Recombinant Monoclonal CD58 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CD58 antibody [EP15041] (AB196648), expandable thumbnail
  • Western blot - Anti-CD58 antibody [EP15041] (AB196648), expandable thumbnail
  • Western blot - Anti-CD58 antibody [EP15041] (AB196648), expandable thumbnail
  • Western blot - Anti-CD58 antibody [EP15041] (AB196648), expandable thumbnail
  • Western blot - Anti-CD58 antibody [EP15041] (AB196648), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Alternative names

CD58, LFA3, Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3

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Rabbit Recombinant Monoclonal CD58 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP15041
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD58 also known as lymphocyte function-associated antigen 3 (LFA-3) is a glycoprotein with a molecular mass of approximately 60 kDa. It is present on the surface of various cell types including antigen-presenting cells epithelial cells and some other leukocytes. This protein serves as a ligand for CD2 which is expressed on T cells and natural killer cells. Through its interaction with CD2 CD58 mediates cell adhesion and plays a role in immunological synapse formation which is essential for effective immune responses.

Biological function summary

Lymphocyte function-associated antigen 3 acts as a significant participant in the immune system. It plays a role in T cell activation and facilitates interactions between T cells and antigen-presenting cells. CD58 forms part of a complex network of cell surface proteins that enhances immune surveillance and response. Its role highlights its importance in adaptive immunity by promoting cell-to-cell communication and activation.

Pathways

CD58 participates in the cell adhesion pathways. It interacts closely with CD2 which is involved in the activation and signaling of T cells. Through this interaction CD58 plays roles in pathways like T cell receptor signaling and immune response. It enhances cell-cell adhesion helping T cells maintain their position during immune surveillance and activation.

Associated diseases and disorders

CD58 relates to conditions such as autoimmune diseases and certain cancers. In autoimmune disorders aberrant expression or function of CD58 may contribute to inappropriate immune activation leading to tissue damage. In cancer CD58 alterations can affect tumor immune evasion influencing how the immune system recognizes and attacks cancer cells. In multiple sclerosis an autoimmune disease the interaction between CD58 and CD2 has been studied providing insights into its role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CD58 antibody [EP15041] (ab196648), expandable thumbnail

    Western blot - Anti-CD58 antibody [EP15041] (ab196648)

    Lanes 1-4: Merged signal (red and green). Green - ab196648 observed at 43 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab196648 Anti-CD58 antibody [EP15041] was shown to specifically react with CD58 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CD58 knockout HeLa cell line ab265947 (knockout cell lysate Human CD58 knockout HeLa cell lysate ab257880) was used. Wild-type and CD58 knockout samples were subjected to SDS-PAGE. ab196648 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CD58 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human CD58 knockout HeLa cell line (Human CD58 knockout HeLa cell line ab265947)

    Lane 3: HAP1 cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 28 kDa

    Observed band size: 43 kDa

  • Western blot - Anti-CD58 antibody [EP15041] (ab196648), expandable thumbnail

    Western blot - Anti-CD58 antibody [EP15041] (ab196648)

    Lanes 1 - 4: Merged signal (red and green). Green - ab196648 observed at 43 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.

    ab196648 was shown to specifically react with CD58 in wild-type HAP1 cells as signal was lost in CD58 knockout cells. Wild-type and CD58 knockout samples were subjected to SDS-PAGE. ab196648 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 40 µg

    Lane 2: CD58 knockout HAP1 whole cell lysate at 40 µg

    Predicted band size: 28 kDa

    Observed band size: 43 kDa

  • Western blot - Anti-CD58 antibody [EP15041] (ab196648), expandable thumbnail

    Western blot - Anti-CD58 antibody [EP15041] (ab196648)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/5000 dilution

    Lane 1: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 65-70 kDa

  • Western blot - Anti-CD58 antibody [EP15041] (ab196648), expandable thumbnail

    Western blot - Anti-CD58 antibody [EP15041] (ab196648)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/1000 dilution

    Lane 1: TF-1 (Human bone marrow erythroleukemia cells) whole cell lysate at 20 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

    Observed band size: 65-70 kDa

  • Western blot - Anti-CD58 antibody [EP15041] (ab196648), expandable thumbnail

    Western blot - Anti-CD58 antibody [EP15041] (ab196648)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed band size : 65-70 (27, 28) kDa.

    All lanes: Western blot - Anti-CD58 antibody [EP15041] (ab196648) at 1/2000 dilution

    Lane 1: SW480 (Human colorectal adenocarcinoma cell line) control at 10 µg

    Lane 2: SW480 (Human colorectal adenocarcinoma cell line) de-glycosylation at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 28 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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