AB133707

Anti-CD59 antibody [EPR6425(2)]

RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Recombinant Monoclonal CD59 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (AB133707)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with ab133707 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Immunohistochemical analysis of CD59 in paraffin embedded Human tonsil tissue labelled with ab133707 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Lane 1 : Wild-type HAP1 whole cell lysate (40 μg)
Lane 2 : CD59 knockout HAP1 whole cell lysate (40 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab133707 observed at 14 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. ab133707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)

Predicted band size: 14 kDa

false

Unknown

Western blot - Anti-CD59 antibody [EPR6425(2)] (AB133707)

All lanes:

Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

BxPC 3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 14 kDa

false

Carrier free

Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD59 antibody [EPR6425(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD59 antibody [EPR6425(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD59 antibody [EPR6425(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD59 antibody [EPR6425(2)]
660 APC

APC Anti-CD59 antibody [EPR6425(2)]
578 PE

PE Anti-CD59 antibody [EPR6425(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD59 antibody [EPR6425(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6425(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.

Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.

See full target information CD59

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003086 PubMed40208872

2025

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Iratxe Zuazo-Gaztelu,David Lawrence,Ioanna Oikonomidi,Scot Marsters,Ximo Pechuan-Jorge,Catarina J Gaspar,David Kan,Ehud Segal,Kevin Clark,Maureen Beresini,Marie-Gabrielle Braun,Joachim Rudolph,Zora Modrusan,Meena Choi,Wendy Sandoval,Mike Reichelt,David C DeWitt,Pekka Kujala,Suzanne van Dijk,Judith Klumperman,Avi Ashkenazi

International journal of molecular sciences 26: PubMed40141433

2025

A Novel Homozygous Missense Variant of Related to Multiple Congenital Anomalies-Hypotonia Seizures Syndrome 3 with Elevated of Serum ALP Level in a Thai Newborn Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Jeerawan Klangjorhor,Natrujee Wiwattanadittakul,Thanapak Jaimalai,Patcharawadee Thongkumkoon,Pitiporn Noisagul,Ratchadaporn Khiaomai,Nutnicha Sirikaew,Nonthanan Moonsan,Arnat Pasena,Pathacha Suksakit,Pimpisa Teeyakasem,Parunya Chaiyawat,Maliwan Tengsujaritkul

Journal of extracellular biology 2:e124 PubMed38938900

2024

Cancer cell invasion alters the protein profile of extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jens C Luoto,Leila S Coelho-Rato,Cecilia Jungarå,Sara H Bengs,Jannica Roininen,John E Eriksson,Lea Sistonen,Eva Henriksson

Pathology, research and practice 247:154519 PubMed37244049

2023

Complement and complement regulatory proteins are upregulated in lungs of COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Ge,Zhui Yu,Xinxin Guo,Ling Li,Ling Ye,Maosong Ye,Jingping Yuan,Chouwen Zhu,Weiguo Hu,Yingyong Hou

Bioactive materials 9:251-265 PubMed34820569

2021

Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yundi Chen,Lixue Wang,Mingfeng Zheng,Chuandong Zhu,Guosheng Wang,Yiqiu Xia,Ethan J Blumenthal,Wenjun Mao,Yuan Wan

Frontiers in cell and developmental biology 4:120 PubMed27857940

2016

Protocol for Biomarker Ratio Imaging Microscopy with Specific Application to Ductal Carcinoma of the Breast.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Clark,Howard R Petty

Scientific reports 6:27039 PubMed27247112

2016

Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Clark,Howard R Petty

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD59 antibody [EPR6425(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Western blot - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Western blot - Anti-CD59 antibody [EPR6425(2)] (AB133707)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise