AB9183

Anti-CD59 antibody [MEM-43/5]

Name: Anti-CD59 antibody [MEM-43/5] (ab9183) | Abcam
Brand: Abcam Limited
SKU: AB9183
Price: 360 USD
Availability: InStock
Rating: 5 (1 reviews)

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What is this?

(1 Review)

(15 Publications)

Anti-CD59 antibody [MEM-43/5] (ab9183) is a mouse monoclonal antibody detecting CD59 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Over 10 publications
- Trusted since 2002

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [MEM-43/5] (AB9183)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [MEM-43/5] (AB9183)

ICC/IF image of ab9183 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (AB9183)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (AB9183)

Immunohistochemistry paraffin embedded sections staining of human skin tissue using ab9183.

Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (AB9183)

Flow Cyt

Lab

Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (AB9183)

Overlay histogram showing Jurkat cells stained with ab9183 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9183, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (AB9183)

IHC image of ab9183 staining CD59 in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab9183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (AB9183)

Flow cytometric analysis of Human Peripheral Blood cells labelling CD59 with ab9183 at 0.6 ug/ml showing separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-43/5

Isotype

IgG2b

Light chain type

unknown

Carrier free

Reacts with

Human, Mouse

Applications

Flow Cyt, WB, IHC-P, IP, ICC/IF

applications

Immunogen

Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information.

P13987

Epitope

The antibody MEM-43/5 reacts with well defined epitope around L33 (see Bodian et al)

Specificity

CD59 antigen (human). MEM-43/5 reacts with well defined epitope (around L33) and does not compete with MEM-43 and many other CD59 antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-4 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "CD59 is GPI-anchored, so we recommend to use a laurylmatoside based lysis buffer or triton base buffer (see Bodian et al; 1% Triton X-100, 1 Âμg/ml leupeptin, 1 Âμg/ml pepstatin A and 1 mM phenlymethylsulphonyl fluoride in PBS), not NP40.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "CD59 is GPI-anchored, so we recommend to use a laurylmatoside based lysis buffer or triton base buffer (see Bodian et al; 1% Triton X-100, 1 Âμg/ml leupeptin, 1 Âμg/ml pepstatin A and 1 mM phenlymethylsulphonyl fluoride in PBS), not NP40.", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CD59 antibody [MEM-43/5] (ab9183) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of CD59?
Anti-CD59 [MEM-43/5] (ab9183) specifically detects a band for CD59 (UniProt: P13987) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-CD59 [MEM-43/5] (ab9183) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.

Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.

See full target information CD59

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0180424 PubMed39846741

2025

Mitochondrial injury and complement dysregulation are drivers of pathological inflammation in viral myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Yasir Mohamud,Amirhossein Bahreyni,Sinwoo Wendy Hwang,Jingfei Carly Lin,Zhihan Claire Wang,Jingchun Zhang,Honglin Luo

Cancer immunology research 10:1475-1489 PubMed36206575

2022

CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Luying Li,Peipei Ding,Xinyue Lv,Suhong Xie,Ling Li,Jianfeng Chen,Danlei Zhou,Xiaochao Wang,Qi Wang,Wei Zhang,Yanqing Xu,Renquan Lu,Weiguo Hu

Reproduction (Cambridge, England) 160:431-445 PubMed32567557

2020

Patterns of MTT reduction in mammalian spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Robert John Aitken,Diatsendoula Gregoratos,Leslie Kutzera,Emma Towney,Minjie Lin,Alexandra Wilkins,Zamira Gibb

Viruses 12: PubMed32276457

2020

HIV Reactivation in Latently Infected Cells With Virological Synapse-Like Cell Contact.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiki Okutomi,Satoko Minakawa,Riku Hirota,Koko Katagiri,Yuko Morikawa

Journal of neuroscience research 98:1498-1513 PubMed32166783

2020

Single-cell RNA sequencing study of retinal immune regulators identified CD47 and CD59a expression in photoreceptors-Implications in subretinal immune regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Liu,Miao Tang,Kevin Harkin,Xuan Du,Chang Luo,Mei Chen,Heping Xu

Molecular genetics and metabolism 130:49-57 PubMed32165008

2020

Homozygous splice-variants in human ARV1 cause GPI-anchor synthesis deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Mariska Davids,Minal Menezes,Yiran Guo,Scott D McLean,Hakon Hakonarson,Felicity Collins,Lisa Worgan,Charles J Billington,Irina Maric,Rebecca Okashah Littlejohn,Tito Onyekweli, Members Of The Udn,David R Adams,Cynthia J Tifft,William A Gahl,Lynne A Wolfe,John Christodoulou,May Christine V Malicdan

Cell death & disease 9:887 PubMed30166523

2018

CD59 is a potential biomarker of esophageal squamous cell carcinoma radioresistance by affecting DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Zhou,Li Chu,Qi Wang,Weixing Dai,Xiaozhou Zhang,Jianfeng Chen,Ling Li,Peipei Ding,Long Zhang,Hongyu Gu,Luying Li,Xinyue Lv,Wei Zhang,Danlei Zhou,Pingzhao Zhang,Guoxiang Cai,Kuaile Zhao,Weiguo Hu

Carbohydrate polymers 180:376-384 PubMed29103517

2017

Stability and bioactivity of chitosan as a transfection agent in primary human cell cultures: A case for chitosan-only controls.

Applications

Unspecified application

Species

Unspecified reactive species

Tanya L Cupino,Billy A Watson,Alan C Cupino,Keiji Oda,Mark G Ghamsary,Salvador Soriano,Wolff M Kirsch

PloS one 12:e0177487 PubMed28542228

2017

Alteration of membrane complement regulators is associated with transporter status in patients on peritoneal dialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Kitterer,Dagmar Biegger,Stephan Segerer,Niko Braun,M Dominik Alscher,Joerg Latus

Human reproduction (Oxford, England) 30:2597-613 PubMed26345691

2015

The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth G Bromfield,R John Aitken,Amanda L Anderson,Eileen A McLaughlin,Brett Nixon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD59 antibody [MEM-43/5]

Immunocytochemistry/ Immunofluorescence - Anti-CD59 antibody [MEM-43/5] (AB9183)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (AB9183)

Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (AB9183)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (AB9183)

Flow Cytometry - Anti-CD59 antibody [MEM-43/5] (AB9183)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise