Anti-CD59 antibody [MEM-43] (ab9182) is a mouse monoclonal antibody detecting CD59 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human.
- Over 20 publications
- Trusted since 2002
Images
![Flow Cytometry - Anti-CD59 antibody [MEM-43] (AB9182), expandable thumbnail](https://content.abcam.com/products/images/cd59-antibody-mem-43-ab9182--flow-cytometry-img422659.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD59 antibody [MEM-43] (AB9182)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (AB9182), expandable thumbnail](https://content.abcam.com/products/images/cd59-antibody-mem-43-ab9182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46159.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (AB9182)")
Publications
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- International journal of molecular sciences 23:2022Bispecific mAb Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Stadlbauer,Peter Andorfer,Gerhard Stadlmayr,Florian Rüker,Gordana Wozniak-KnoppPubMed 35563599
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 22:46-572021Kidney transplantation from triple-knockout pigs expressing multiple human proteins in cynomolgus macaques.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Ma,Takayuki Hirose,Grace Lassiter,Hajime Sasaki,Ivy Rosales,Taylor M Coe,Charles G Rickert,Rudy Matheson,Robert B Colvin,Wenning Qin,Yinan Kan,Jacob V Layer,Violette B Paragas,Kathryn Stiede,Katherine C Hall,Michele E Youd,Luis M Queiroz,William F Westlin,Michael Curtis,Luhan Yang,James F Markmann,Tatsuo KawaiPubMed 34331749
- Cellular and molecular life sciences : CMLS 78:3637-36562021The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnette Brandel,Sahaja Aigal,Simon Lagies,Manuel Schlimpert,Ana Valeria Meléndez,Maokai Xu,Anika Lehmann,Daniel Hummel,Daniel Fisch,Josef Madl,Thorsten Eierhoff,Bernd Kammerer,Winfried RömerPubMed 33555391
- BioMed research international 2020:27534142020Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Yan,Yan Li,Shaoju Min,Peng Zhang,Bin Xu,Zhen Wang,Wei Zhang,Jiasheng Chen,Guangheng Luo,Chunxiao LiuPubMed 32337233
- Journal of Cancer 10:4165-41772019iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Yan et. al.PubMed 31413735
- Journal of cellular and molecular medicine 23:5956-59692019Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Chen et. al.PubMed 31293077
- Cell 177:1738-1756.e232019Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph Mathew Kalappurakkal et. al.PubMed 31104842
- Oncology letters 15:9061-90682018Effect of membrane-bound complement regulatory proteins on tumor cell sensitivity to complement-dependent cytolysis triggered by heterologous expression of the α-gal xenoantigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Juan Liao,Ya-Jun Yang,Zhu Wang,Feng Qin,Sheng-Ming Zhu,Hong Zheng,Yan-Ping WangPubMed 29805637
- Oncology letters 14:2961-29692017CD55 and CD59 expression protects HER2-overexpressing breast cancer cells from trastuzumab-induced complement-dependent cytotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Wang,Ya-Jun Yang,Zhu Wang,Juan Liao,Mei Liu,Xiao-Rong Zhong,Hong Zheng,Yan-Ping WangPubMed 28928834
- Oncology letters 12:1507-15122016Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13987
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Reactivity data
Select an application| IP | IHC | Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected | Tested | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-4.00000 µg/mL | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use under non-reducing conditions. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Alternative names
CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL
Recommended products
Anti-CD59 antibody [MEM-43] (ab9182) is a mouse monoclonal antibody detecting CD59 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Human.
- Over 20 publications
- Trusted since 2002
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13987
- Clone number
- MEM-43
- Purification technique
- Affinity purification Protein A
- Specificity
CD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (W40, R53).
- Light chain type
- unknown
- Concentration
- Purification notes
Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
Anti-CD59 antibody [MEM-43] (ab9182) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of CD59?
Anti-CD59 [MEM-43] (ab9182) specifically detects a band for CD59 (UniProt: P13987) at a molecular weight of 14kDa.
Trusted by the scientific community
Anti-CD59 [MEM-43] (ab9182) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [MEM-43] also available for your convenience: ab9182, FITC - FITC Anti-CD59 antibody [MEM-43] ab18237, Biotin - Biotin Anti-CD59 antibody [MEM-43] ab28114, PE - PE Anti-CD59 antibody [MEM-43] ab36421, APC - APC Anti-CD59 antibody [MEM-43] ab36467, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] ab187769
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
- Biological function summary
One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.
- Pathways
CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.
- Associated diseases and disorders
Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Flow Cytometry - Anti-CD59 antibody [MEM-43] (ab9182), expandable thumbnail](https://content.abcam.com/products/images/cd59-antibody-mem-43-ab9182--flow-cytometry-img422659.jpg "Flow Cytometry - Anti-CD59 antibody [MEM-43] (ab9182)")
Flow Cytometry - Anti-CD59 antibody [MEM-43] (ab9182)
Flow cytometric analysis of Human Peripheral Blood cells labelling CD59 with ab9182 at 0.3 ug/ml showing separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182), expandable thumbnail](https://content.abcam.com/products/images/cd59-antibody-mem-43-ab9182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46159.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)
Immunohistochemistry parafin embedded sections staining of huam palacenta tissue using ab9182 with a concentration of 10μg / ml
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