Rabbit Polyclonal CD5L/CT-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications.
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- BMC cancer 23:5492023Characterization and clinical verification of immune-related genes in hepatocellular carcinoma to aid prognosis evaluation and immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin Qu,Fenghao Sun,Yichen Hou,Haoran Qi,Xiaorong Sun,Ligang XingPubMed 37322434
- Chinese medical journal 136:556-5642023Role of CD5 molecular-like on hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaowei Gao,Xianan Wu,Lan Yang,Chong Liu,Xi Wang,Huiping Wang,Ke DongPubMed 36939243
- Molecular & cellular proteomics : MCP 21:1004062022Mass Spectrometry Identification of Biomarkers in Extracellular Vesicles From Plasmodium vivax Liver Hypnozoite Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelisa Gualdrón-López,Miriam Díaz-Varela,Gigliola Zanghi,Iris Aparici-Herraiz,Ryan W J Steel,Carola Schäfer,Pol Cuscó,Vorada Chuenchob,Niwat Kangwangransan,Zachary P Billman,Tayla M Olsen,Juan R González,Wanlapa Roobsoong,Jetsumon Sattabongkot,Sean C Murphy,Sebastian A Mikolajczak,Eva Borràs,Eduard Sabidó,Carmen Fernandez-Becerra,Erika L Flannery,Stefan H I Kappe,Hernando A Del PortilloPubMed 36030044
- Scientific reports 12:133442022Extracellular vesicles derived from GMSCs stimulated with TNF-α and IFN-α promote M2 macrophage polarization via enhanced CD73 and CD5L expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukari Watanabe,Takao Fukuda,Chikako Hayashi,Yuki Nakao,Masaaki Toyoda,Kentaro Kawakami,Takanori Shinjo,Misaki Iwashita,Hiroaki Yamato,Karen Yotsumoto,Takaharu Taketomi,Takeshi Uchiumi,Terukazu Sanui,Fusanori NishimuraPubMed 35922474
- Frontiers in cell and developmental biology 10:8122442022Validation of Effective Extracellular Vesicles Isolation Methods Adapted to Field Studies in Malaria Endemic Regions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Zoia,Bibin Yesodha Subramanian,Klara Kristin Eriksson,Meera Sruthi Ravi,Shekoofeh Yaghmaei,Isabelle Fellay,Brigitte Scolari,Michael Walch,Pierre-Yves MantelPubMed 35652104
- Frontiers in endocrinology 12:8306912022Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Wang,Xiaoyang Fei,Hongyan Zhang,Wenjing Zhou,Zhaojun Cheng,Ying FengPubMed 35185790
- Cell death discovery 7:3422021CD5L deficiency attenuate acetaminophen-induced liver damage in mice via regulation of JNK and ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjing Li,Tao Ling,Fengmeng Teng,Chao Hu,Zhongping Su,Chen Zhang,Xiang Li,Ting Zhao,Xianmin Mu,Yingchang Li,Jinshun Pan,Qiang YouPubMed 34750342
- Nature communications 11:27612020Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaruka Toda et. al.PubMed 32487994
- Translational neurodegeneration 8:312019Exploratory study on microRNA profiles from plasma-derived extracellular vesicles in Alzheimer's disease and dementia with Lewy bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Gámez-Valero et. al.PubMed 31592314
- Scientific reports 8:174872018Targeted-pig trial on safety and immunogenicity of serum-derived extracellular vesicles enriched fractions obtained from Porcine Respiratory and Reproductive virus infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio Montaner-Tarbes,Elena Novell,Vicens Tarancón,Francesc E Borrás,Maria Montoya,Lorenzo Fraile,Hernando A Del PortilloPubMed 30504834
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 20 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (By similarity). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983).
Alternative names
API6, UNQ203/PRO229, CD5L, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, CT-2, IgM-associated peptide, SP-alpha, hAIM
Recommended products
Rabbit Polyclonal CD5L/CT-2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD5L also known as CT-2 or CD5 antigen-like is a secreted glycoprotein with a molecular mass of approximately 50 kDa. This protein expresses mainly in liver and to a lesser extent in spleen lymph nodes and other immune-related tissues. The CD5L protein acts as a scavenger receptor cysteine-rich (SRCR) superfamily member which participates in pathogen recognition and clearance. Researchers often measure CD5L levels using CD5L ELISA kits providing quantitative analysis of this biomarker in various biological samples.
- Biological function summary
CD5L influences several immune processes modulating macrophage function and lipid metabolism. It plays a role within a complex system transforming macrophages into an anti-inflammatory phenotype. This helps the clearance of apoptotic cells and prevents tissue damage during inflammation. CD5L also impacts cholesterol homeostasis contributing to lipid clearance and influencing foam cell formation which serves a significant role in atherosclerosis development.
- Pathways
This protein integrates into the lipid metabolism pathway and the immune regulation pathway. Within the lipid metabolism pathway interaction with proteins such as apolipoprotein AI demonstrates its role in controlling cholesterol and triglyceride levels. In the immune regulation pathway CD5L collaborates with other immune-related proteins including CD36 and TLR2 to regulate immune response and inflammation affecting macrophage activation and phagocytosis.
- Associated diseases and disorders
CD5L associates with atherosclerosis and systemic lupus erythematosus (SLE). Researchers link elevated CD5L levels with atherosclerosis due to its ability to drive macrophage transformation into foam cells linking it with cardiovascular diseases. In systemic lupus erythematosus CD5L interacts with protein serum amyloid A (SAA) exacerbating the inflammatory processes associated with autoimmune disorders. Understanding CD5L's role may provide insights for developing therapeutic strategies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CD5L/CT-2 antibody (ab45408)
Lane 1: Western blot - Anti-CD5L/CT-2 antibody (ab45408) at 1 µg/mL
Lane 2: Western blot - Anti-CD5L/CT-2 antibody (ab45408) at 2 µg/mL
Lane 3: Western blot - Anti-CD5L/CT-2 antibody (ab45408) at 4 µg/mL
All lanes: human lymph node tissue lysate
Predicted band size: 38 kDa
Observed band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody (ab45408)
ab45408 at 2μg/ml staining CD5L/CT-2 in human lymph node tissue by IHC
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