Anti-CD5L/CT-2 antibody [EPR29363-540]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD5L/CT-2 with ab323268 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on kupffer cells of human liver. The section was incubated with ab323268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver cancer tissue staining CD5L with ab323268 at a 1/2000 dilution (0.246 μg/ml) and CD68 with ab213363 at 1/500 dilution (1.26 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-CD5L (green; Opal™520) and anti-CD68 (magenta; Opal™690) on human liver cancer.
Panel B : anti-CD5L staining Kupffer cells in human liver cancer.
Panel C : anti-CD68 staining Kupffer cells in human liver cancer.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323268 and ab213363 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining CD5L with ab323268 at a 1/2000 dilution (0.246 μg/ml) and CD68 with ab213363 at 1/500 dilution (1.26 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-CD5L (green; Opal™520) and anti-CD68 (magenta; Opal™690) on human spleen.
Panel B : anti-CD5L staining Kupffer cells in human spleen.
Panel C : anti-CD68 staining Kupffer cells in human spleen.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323268 and ab213363 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining CD5L with ab323268 at a 1/2000 dilution (0.246 μg/ml) and CD68 with ab213363 at 1/500 dilution (1.26 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-CD5L (green; Opal™520) and anti-CD68 (magenta; Opal™690) on human liver.
Panel B : anti-CD5L staining Kupffer cells in human liver.
Panel C : anti-CD68 staining Kupffer cells in human liver.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323268 and ab213363 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CD5L/CT-2 with ab323268 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on some scattered cells in human testis. The section was incubated with ab323268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD5L/CT-2 with ab323268 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on human skeletal muscle. The section was incubated with ab323268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD5L/CT-2 with ab323268 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human spleen. The section was incubated with ab323268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue labeling CD5L/CT-2 with ab323268 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of hepatocellular carcinoma. The section was incubated with ab323268 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis (PMID : 10651944)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD5L/CT-2 antibody [EPR29363-540] (ab323268) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 40 µg

Lane 2:

Human plasma lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-CD5L/CT-2 antibody [EPR29363-540] (AB323268)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum, skeletal muscle (PMID : 10651944)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD5L/CT-2 antibody [EPR29363-540] (ab323268) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 6s