Rabbit Recombinant Monoclonal CD5L/CT-2 antibody. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Mouse, Mouse samples.
Images
![Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--multiplex-immunohistochemistry-img446748.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446726.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446725.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446724.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446723.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (AB324193)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | mIHC | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Not recommended | Not recommended |
| Transfected cell line - Mouse | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Target data
Function
Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis (PubMed:26048980). Able to inhibit lipid droplet size in adipocytes (PubMed:20519120, PubMed:22579686). Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA) (PubMed:20519120). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease (PubMed:21730133). Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC) (PubMed:22579686, PubMed:26607793). Acts as a key regulator of metabolic switch in T-helper Th17 cells (PubMed:26607793, PubMed:26607794). Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation (PubMed:26607793). CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes (PubMed:26607793). Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (PubMed:23562157). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:16054063, PubMed:9892623). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (By similarity).
Alternative names
Aim, Api6, Cd5l, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, Apoptosis inhibitory 6, SP-alpha, mAIM
Rabbit Recombinant Monoclonal CD5L/CT-2 antibody. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Mouse, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR29364-21
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--multiplex-immunohistochemistry-img446748.jpg "Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Multiplex immunohistochemistry - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-CD5L/CT-2 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining CD5L/CT-2 with ab324193 at a 1:500 (0.958 ug/ml) dilution, Anti-CD68 antibody [EPR23917-164] ab283654 anti-CD68 used at 1:100 (4.43 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL57] ab237721 anti-CD3 used at a 1:2000 (0.25 ug/ml) dilution.
Panel A: merged staining of anti-CD5 antigen-like (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on mouse liver.
Panel B: anti-CD5 antigen-like staining macrophages in mouse liver.
Panel C: anti-CD68 staining macrophages in mouse liver.
Panel D: anti-CD3 staining T lymphocytes in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of ab324193, Anti-CD68 antibody [EPR23917-164] ab283654 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446726.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-CD5L/CT-2 antibody
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse CD5L expression vector containing a his tag. (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling CD5L/CT-2 with ab324193 at 1/2000 (0.958 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a mouse CD5L expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND™ RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446725.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cardiac muscle using rabbit Anti-CD5L/CT-2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD5L/CT-2 with ab324193 at 1/500 (0.958 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on mouse cardiac muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446724.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse skeletal muscle using rabbit Anti-CD5L/CT-2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD5L/CT-2 with ab324193 at 1/500 (0.958 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: no staining on mouse skeletal muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446723.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-CD5L/CT-2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD5L/CT-2 with ab324193 at 1/500 (0.958 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on macrophages in mouse spleen. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446722.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)
CD5L/CT-2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-CD5L/CT-2 antibody
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD5L/CT-2 with ab324193 at 1/500 (0.958 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on Kupffer cells of mouse liver (PMID: 34750342). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193), expandable thumbnail](https://content.abcam.com/products/images/cd5l-ct-2-antibody-epr29364-21-ab324193--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446722.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5L/CT-2 antibody [EPR29364-21] (ab324193)")
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