Mouse Monoclonal CD6/T12 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing T-cell differentiation antigen CD6 protein.
Images
![Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunocytochemistry-img60472.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)")
![Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--flow-cytometry-img54072.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37568.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81399.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (AB213007)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing T-cell differentiation antigen CD6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P30203
Reactivity data
Select an application| IHC-P | ICC | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Primary incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24584089, PubMed:24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).
Alternative names
CD6, T-cell differentiation antigen CD6, T12, TP120
Recommended products
Mouse Monoclonal CD6/T12 antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing T-cell differentiation antigen CD6 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing T-cell differentiation antigen CD6 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P30203
- Carrier free
- Yes
- Clone number
- 3F7B5
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
ab213007 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.
- Biological function summary
CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.
- Pathways
CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.
- Associated diseases and disorders
CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
![Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunocytochemistry-img60472.jpg "Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)")
Immunocytochemistry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)
Immunofluorescence staining of MOLT-4 cells labeling CD6/T12 with ab213007 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
![Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--flow-cytometry-img54072.jpg "Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)")
Flow Cytometry - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)
This image was produced using Anti-CD6/T12 antibody [3F7B5] ab193341, the same clone but in a different formulation. Flow Cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cells staining CD6/T12 using ab213007 at 2ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img37568.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)
Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 μg/mL.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007), expandable thumbnail](https://content.abcam.com/products/images/cd6-t12-antibody-3f7b5-bsa-and-azide-free-ab213007--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81399.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [3F7B5] - BSA and Azide free (ab213007)
Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 μg/mL.
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