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AB270302

Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD6/T12 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human T-cell differentiation antigen CD6.

View Alternative Names

CD6, T-cell differentiation antigen CD6, T12, TP120

2 Images
Flow Cytometry - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (AB270302)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (AB270302)

Flow cytometric analsysis of MOLT-4 (Human lymphoblastic leukemia cell line) cells labeling CD6/T12 using ab270271 at 2μg/10^6 cells followed by the goat anti-rabbit IG-CF488 (blue); isotope control (red).

This image was produced using the same antibody clone but in a different formulation ab270271, PBS, BSA and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (AB270302)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (AB270302)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD6/T12 using ab270271 at 2μg/ml in immunohistochemical analyisis.

This image was produced using the same antibody clone but in a different formulation ab270271, PBS, BSA and sodium azide.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

C6/2884R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human T-cell differentiation antigen CD6.

P30203

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab270302 is a carrier free version of ab270271.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.
Biological function summary

CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.

Pathways

CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.

CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed : 15048703, PubMed : 15294938, PubMed : 16352806, PubMed : 16914752, PubMed : 24584089, PubMed : 24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed : 24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed : 15294938, PubMed : 16352806, PubMed : 16914752). Contributes to the formation and maturation of the immunological synapse (PubMed : 15294938, PubMed : 16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed : 17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed : 17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed : 17601777).
See full target information T-cell differentiation antigen CD6
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Product promise

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