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Rabbit Recombinant Monoclonal CD6/T12 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunoprecipitation - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (AB247795), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (AB247795), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (AB247795), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (AB247795), expandable thumbnail
  • Western blot - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (AB247795), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

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      Target data

      Function

      Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24945728, PubMed:24584089). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).

      Alternative names

      T-cell differentiation antigen CD6, T12, TP120, CD6

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      Rabbit Recombinant Monoclonal CD6/T12 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

      Alternative names

      T-cell differentiation antigen CD6, T12, TP120, CD6

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR4057

      Purification technique

      Affinity purification Protein A

      Concentration
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      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab247795 is the carrier-free version of Anti-CD6/T12 antibody [EPR4057] ab109217.

      Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.

      Biological function summary

      CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.

      Pathways

      CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.

      Associated diseases and disorders

      CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Immunoprecipitation - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795), expandable thumbnail

        Immunoprecipitation - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)

        Purified Anti-CD6/T12 antibody [EPR4057] ab109217 at 1:20 dilution (0.6μg) immunoprecipitating CD6/T12 in HuT-78 whole cell lysate.
        Lane 1 (input): HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10μg
        Lane 2 (+): Anti-CD6/T12 antibody [EPR4057] ab109217 + HuT-78 whole cell lysate.
        Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD6/T12 antibody [EPR4057] ab109217 in HuT-78 whole cell lysate.
        Blocking Buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM/TBST.
        Observed band size: 105-130 kDa

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD6/T12 antibody [EPR4057] ab109217).

        All lanes: Immunoprecipitation - Anti-CD6/T12 antibody [EPR4057] (Anti-CD6/T12 antibody [EPR4057] ab109217)

        Predicted band size: 72 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)

        Immunocytochemistry/ Immunofluorescence analysis of Human PBMC (Human primary peripheral blood mononuclear cell) cells labeling CD6/T12 with Purified Anti-CD6/T12 antibody [EPR4057] ab109217 at 1:50 dilution (2.3 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD6/T12 antibody [EPR4057] ab109217).

      • Flow Cytometry (Intracellular) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)

        Intracellular Flow Cytometry analysis of HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) cells labeling CD6/T12 with Purified Anti-CD6/T12 antibody [EPR4057] ab109217 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD6/T12 antibody [EPR4057] ab109217).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD6/T12 with purified Anti-CD6/T12 antibody [EPR4057] ab109217 at 1/1000 dilution (0.115 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
        The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD6/T12 antibody [EPR4057] ab109217).

      • Western blot - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795), expandable thumbnail

        Western blot - Anti-CD6/T12 antibody [EPR4057] - BSA and Azide free (ab247795)

        The molecular weight of CD6 ranges from 105 to 130 kDa depending on its degree of phosphorylation (PMID: 17601777).

        Blocking/Diluting buffer: 5% NFDM/TBST

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD6/T12 antibody [EPR4057] ab109217).

        All lanes: Western blot - Anti-CD6/T12 antibody [EPR4057] (Anti-CD6/T12 antibody [EPR4057] ab109217) at 1/5000 dilution

        All lanes: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 15 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 72 kDa

        Observed band size: 105-130 kDa

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