Anti-CD6/T12 antibody [MEM-98]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Flow cytometric analysis of human peripheral whole blood labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Flow cytometric analysis of human CD6 positive lymphocytes (red-filled) / neutrophil granulocytes (black-dashed) from peripheral whole blood cells labeling CD6/T12 with ab9181 at 2µg/ml. Secondary used is a goat anti-mouse-APC.

• WB

Supplier Data

Western blot - Anti-CD6/T12 antibody [MEM-98] (AB9181)

Nitrocellulose membrane blot was probed with ab9181 used at 1 µg/ml, followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

CD6 molecules were detected at ~100 kDa in Molt-4 cell line under non-reducing conditions.

All lanes:

Western blot - Anti-CD6/T12 antibody [MEM-98] (ab9181) at 1 µg/mL

Lane 1:

Molt-4 whole cell extracts, reducing

Lane 2:

HeLa whole cell extracts, reducing

Lane 3:

Molt-4 whole cell extracts, non-reducing

Lane 4:

HeLa whole cell extracts, non-reducing

Secondary

All lanes:

IRDye 800CW Goat-anti-Mouse IgG

Predicted band size: 72 kDa

false