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AB6630

Anti-CD62E antibody [1.2B6]

3

(3 Reviews)

|

(11 Publications)

Mouse Monoclonal CD62E antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Full Length Protein corresponding to Human SELE.

View Alternative Names

CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

1 Images
Flow Cytometry - Anti-CD62E antibody [1.2B6] (AB6630)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62E antibody [1.2B6] (AB6630)

ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1.2B6

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human SELE. The exact immunogen used to generate this antibody is proprietary information.

P16581

Specificity

This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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You may be interested in:

AB303747

Human CD62E ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information SELE
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 53:400-408 PubMed28950359

2017

Recipient treatment with acetylcholinesterase inhibitor donepezil attenuates primary graft failure in rats through inhibiting post-transplantational donor heart ischaemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yuan,Xiao Teng,Yin Wang,Yuntai Yao

The Journal of neuroscience : the official journal 37:1797-1806 PubMed28093478

2017

Long Noncoding RNA Malat1 Regulates Cerebrovascular Pathologies in Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejing Zhang,Xuelian Tang,Kai Liu,Milton H Hamblin,Ke-Jie Yin

PloS one 8:e78441 PubMed24205233

2013

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing.

Applications

Unspecified application

Species

Unspecified reactive species

Claire L Allen,Gerry Clare,Elizabeth A Stewart,Matthew J Branch,Owen D McIntosh,Megha Dadhwal,Harminder S Dua,Andrew Hopkinson

Molecular medicine reports 7:1251-6 PubMed23403989

2013

shRNAs targeting high-mobility group box-1 inhibit E-selectin expression via homeobox A9 in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Juan Zhang,Zheng-Gang Luan,Xiao-Chun Ma

The Journal of pharmacology and experimental thera 334:260-8 PubMed20378716

2010

NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion.

Applications

WB

Species

Human

Hongfei Zhou,Donna Dehn,Jadwiga K Kepa,David Siegel,Devon E Scott,Wei Tan,David Ross

Investigative ophthalmology & visual science 50:4162-72 PubMed19324848

2009

Molecular profiling of conjunctival epithelial side-population stem cells: atypical cell surface markers and sources of a slow-cycling phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

M A Murat Akinci,Helen Turner,Maria Taveras,Alex Barash,Zheng Wang,Peter Reinach,J Mario Wolosin

World journal of gastroenterology 12:4529-35 PubMed16874866

2006

Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Tso Lin,Shih-Hwa Chiou,Chung-Lan Kao,Yi-Ming Shyr,Chien-Jen Hsu,Yih-Wen Tarng,Larry L-T Ho,Ching-Fai Kwok,Hung-Hai Ku

European journal of immunology 29:1551-60 PubMed10359109

1999

Characterization of an apparently conserved epitope in E- and P-selectin identified by dual-specific monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

K Goda,T Tanaka,M Monden,M Miyasaka

The American journal of physiology 266:H278-90 PubMed7508207

1994

Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

E T Keelan,S T Licence,A M Peters,R M Binns,D O Haskard

Journal of immunology (Baltimore, Md. : 1950) 146:521-8 PubMed1702804

1991

Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

U Kyan-Aung,D O Haskard,R N Poston,M H Thornhill,T H Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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