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AB47075

Anti-CD62E antibody [B-P7] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal CD62E antibody. Carrier free. Suitable for Flow Cyt and reacts with Transfected cell line - Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing SELE protein.

View Alternative Names

CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

1 Images
Flow Cytometry - Anti-CD62E antibody [B-P7] - BSA and Azide free (AB47075)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD62E antibody [B-P7] - BSA and Azide free (AB47075)

A typical staining pattern with ab47075 of ELAM-1 transfected CHO cell line

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-P7

Isotype

IgG1

Light chain type

lambda

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing SELE protein. The exact immunogen used to generate this antibody is proprietary information.

P16581

Specificity

Recognises soluble Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1), E-selectin

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Alternative Products

Primary Antibodies

AB2497

Anti-CD62E antibody [UZ6]

Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (AB2497)

  • 4
  • 1
Primary Antibodies

AB6630

Anti-CD62E antibody [1.2B6]

Flow Cytometry - Anti-CD62E antibody [1.2B6] (AB6630)

  • 3
  • 3
Primary Antibodies

AB185698

Anti-CD62E antibody

Western blot - Anti-CD62E antibody (AB185698)

  • 3
  • 1

Complementary Products

Assay Kits

AB303747

Human CD62E ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 16
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

  • 5
  • 1
Primary Antibodies

AB169545

Anti-CD105 antibody [EPR10145-12]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (AB169545)

  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.3 - 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information SELE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:688 PubMed29449677

2018

USP35 regulates mitotic progression by modulating the stability of Aurora B.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyoung Park,Mi-Sun Kwon,Eunice EunKyeong Kim,Hyunsook Lee,Eun Joo Song
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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