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AB309221

Anti-CD62E antibody [EPR25616-144] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CD62E antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

1 Images
Sandwich ELISA - Anti-CD62E antibody [EPR25616-144] - BSA and Azide free (Detector) (AB309221)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD62E antibody [EPR25616-144] - BSA and Azide free (Detector) (AB309221)

Sandwich ELISA of ab309262 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25616-144

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information SELE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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