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AB300558

Anti-CD62E antibody [EPR25616-62] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD62E antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

2 Images
Flow Cytometry (Intracellular) - Anti-CD62E antibody [EPR25616-62] - BSA and Azide free (AB300558)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD62E antibody [EPR25616-62] - BSA and Azide free (AB300558)

This data was developed using ab300557, the same antibody clone in a different buffer formulation. Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell) treated with 20ng/ml IL-lbeta for 4 hours (Right) / Untreated control (Left) cells labelling CD62E with ab300557 at 1/500 dilution (0.1ug)/ Right and Left compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [EPR25616-62] - BSA and Azide free (AB300558)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [EPR25616-62] - BSA and Azide free (AB300558)

This data was developed using ab300557, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling CD62E with ab300557 at 1/50 (9.36 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased cytoplasmic and membranous staining in HUVEC cells treated with interleukin-lbeta (20 ng/ml) for 4 h (PMID : 16715142) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Unconjugated

    Anti-CD62E antibody [EPR25616-62]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD62E antibody [EPR25616-62]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD62E antibody [EPR25616-62]

  • 578 PE

    PE Anti-CD62E antibody [EPR25616-62]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25616-62

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB303747

Human CD62E ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 16
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB282575

Anti-ICAM1 antibody [EPR24639-3]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR24639-3] (AB282575)

  • 5
  • 1
Primary Antibodies

AB169545

Anti-CD105 antibody [EPR10145-12]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (AB169545)

  • 4
  • 3
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information SELE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com