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Rabbit Recombinant Monoclonal CD62L antibody. Suitable for Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (AB253241), expandable thumbnail
  • Western blot - Anti-CD62L antibody [EPR17012-35] (AB253241), expandable thumbnail
  • Western blot - Anti-CD62L antibody [EPR17012-35] (AB253241), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IFIHC-Fr
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/60

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed:1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD62L antibody. Suitable for Flow Cyt, WB and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17012-35

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab253241 (EPR17012-35) is the detector of the ssELISA kit Mouse L-Selectin ELISA Kit (CD62L) ab199085.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

Associated diseases and disorders

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (ab253241), expandable thumbnail

    Flow Cytometry - Anti-CD62L antibody [EPR17012-35] (ab253241)

    Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253241 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD4 and BV421-conjugated anti-CD44. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

    Gated on viable CD4+ T cells.

  • Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241), expandable thumbnail

    Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241)

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Mouse lymph node lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa

    Observed band size: 75-100 kDa

    Exposure time: 136s

  • Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241), expandable thumbnail

    Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241)

    Lanes 1 - 2: Western blot - Anti-CD62L antibody [EPR17012-35] (ab253241) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (Anti-CD62L antibody [EPR17012-35] - BSA and Azide free ab254204) at 1/1000 dilution

    Lane 1: Mouse serum tissue lysate unboiled at 20 µg

    Lane 2: Mouse serum tissue lysate boiled at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 75 kDa

    Exposure time: 20s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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