Anti-CD62L antibody [EPR17012-35] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253241 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with Alexa Fluor^® 647-conjugated anti-CD4 and BV421-conjugated anti-CD44. While CD4⁺ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

Gated on viable CD4⁺ T cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253241).

• WB

Supplier Data

Western blot - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

This data was developed using the same antibody clone in a different buffer formulation (ab253241).

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 12165530).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD62L antibody [EPR17012-35] (<a href='/en-us/products/primary-antibodies/cd62l-antibody-epr17012-35-ab253241'>ab253241</a>) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 75-100 kDa

false

Exposure time: 136s

• WB

Supplier Data

Western blot - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (AB254204)

This data was developed using the same antibody clone in a different buffer formulation (ab253241).

For better using this antibody, we recommend not to boil your sample.

Blocking and diluting buffer : 5% NFDM /TBST.

Lanes 1 - 2:

Western blot - Anti-CD62L antibody [EPR17012-35] (<a href='/en-us/products/primary-antibodies/cd62l-antibody-epr17012-35-ab253241'>ab253241</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-CD62L antibody [EPR17012-35] - BSA and Azide free (ab254204) at 1/1000 dilution

Lane 1:

Mouse serum unboiled at 20 µg

Lane 2:

Mouse serum boiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 20s