Anti-CD62L antibody [EPR30513-525]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody [EPR30513-525] (AB324590)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD62L with ab324590 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody [EPR30513-525] (AB324590)

Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkins Lymphoma tissue labeling CD62L with ab324590 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human Non-Hodgkins Lymphoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody [EPR30513-525] (AB324590)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human CD62L (L-selectin) expression vector containing a his tag. (B) HEK-293T cells transfected with a human CD62E (E-selectin) expression vector containing a his tag. (C) HEK-293T cells transfected with a human CD62P (P-selectin) expression vector containing a his tag. (D) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling CD62L with ab324590 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a human CD62L (L-selectin) expression vector containing a his tag, no staining on (B) HEK-293T cells transfected with a human CD62E (E-selectin) expression vector containing a his tag, (C) HEK-293T cells transfected with a human CD62P (P-selectin) expression vector containing a his tag and (D) HEK-293T cells transfected with empty vector containing a his tag.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody [EPR30513-525] (AB324590)

Immunohistochemical analysis of paraffin-embedded (A) Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cell pellet, (B) HeLa (human cervical adenocarcinoma epithelial cell) cell pellet tissue labeling CD62L with ab324590 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Jurkat cell pellet, no staining on (B) HeLa cell pellet.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CD62L antibody [EPR30513-525] (AB324590)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CD62L is a glycoprotein of approximately 80-100 kDa and is detected as a 40 kDa band after treatment with the deglycosylation enzyme Peptide : N-glycosidase F (PNGase F).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD62L antibody [EPR30513-525] (ab324590) at 1/1000 dilution

Lane 1:

Untreated Jurkat whole cell lysate  at 20 µg

Lane 2:

Jurkat lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,80 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-CD62L antibody [EPR30513-525] (AB324590)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa, human liver

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD62L antibody [EPR30513-525] (ab324590) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human tonsil tissue lysate  at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 26s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62L antibody [EPR30513-525] (AB324590)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD62L with ab324590 at 1/100 (4.88 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins