AB119834

Anti-CD62L antibody [MEL-14]

Recombinant
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(8 Publications)

Rat Recombinant Monoclonal CD62L antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD62L, Lnhr, Ly-22, Ly22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] (AB119834)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] (AB119834)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling CD62L with ab119834 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse spleen cells is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab119834 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.

-ve control 2 : ab179513 at 1/200 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647 and anti-CD3 conjugated to PerCP.
Gated on viable CD3+ T cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

redFluor™ 710

redFluor™ 710 Anti-CD62L antibody [MEL-14]
778 PE/Cy7®

PE/Cy7® Anti-CD62L antibody [MEL-14]
Biotin

Biotin Anti-CD62L antibody [MEL-14]
519 FITC

FITC Anti-CD62L antibody [MEL-14]
660 APC

APC Anti-CD62L antibody [MEL-14]
Carrier free

Anti-CD62L antibody [MEL-14] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MEL-14

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab119834 reacts with mouse CD62L, a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was switched from a hybridoma to a recombinant production format on 25^th October 2021.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.

Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed : 1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).

See full target information Sell

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 23:518-531 PubMed35354953

2022

Neutrophils direct preexisting matrix to initiate repair in damaged tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Fischer,Juliane Wannemacher,Simon Christ,Tim Koopmans,Safwen Kadri,Jiakuan Zhao,Mahesh Gouda,Haifeng Ye,Martin Mück-Häusl,Peter W Krenn,Hans-Günther Machens,Reinhard Fässler,Philipp-Alexander Neumann,Stefanie M Hauck,Yuval Rinkevich

Biomaterials 242:119932 PubMed32169772

2020

Nanosponges of circulating tumor-derived exosomes for breast cancer metastasis inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Ye,Kaiyuan Wang,Qi Lu,Jian Zhao,Menglin Wang,Qiming Kan,Haotian Zhang,Yongjun Wang,Zhonggui He,Jin Sun

eLife 3: PubMed25347065

2014

CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle A Linterman,Alice E Denton,Devina P Divekar,Ilona Zvetkova,Leanne Kane,Cristina Ferreira,Marc Veldhoen,Simon Clare,Gordon Dougan,Marion Espéli,Kenneth G C Smith

Journal of immunology (Baltimore, Md. : 1950) 177:5-9 PubMed16785489

2006

A cell-surface molecule involved in organ-specific homing of lymphocytes. 1983.

Applications

Unspecified application

Species

Unspecified reactive species

W Michael Gallatin,Irving L Weissman,Eugene C Butcher

Blood 103:1534-41 PubMed14551132

2003

Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease.

Applications

Unspecified application

Species

Unspecified reactive species

Benny J Chen,Xiuyu Cui,Gregory D Sempowski,Congxiao Liu,Nelson J Chao

Journal of immunology (Baltimore, Md. : 1950) 168:2659-66 PubMed11884430

2002

L-selectin is not required for T cell-mediated autoimmune diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Randall H Friedline,Carmen P Wong,Douglas A Steeber,Thomas F Tedder,Roland Tisch

Infection and immunity 70:27-35 PubMed11748160

2001

Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection.

Applications

Unspecified application

Species

Unspecified reactive species

Stuart I Mannering,Christina Cheers

Journal of virology 73:9161-9 PubMed10516023

1999

Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo.

Applications

Flow Cyt

Species

Mouse

P Brawand,J C Cerottini,H R MacDonald

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD62L antibody [MEL-14]

Immunocytochemistry/ Immunofluorescence - Anti-CD62L antibody [MEL-14] (AB119834)

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

Flow Cytometry - Anti-CD62L antibody [MEL-14] (AB119834)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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