AB59738

Anti-P-Selectin (CD62P) antibody

(1 Review)

(10 Publications)

Rabbit Polyclonal CD62P antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Selp.

View Alternative Names

CD62P, GMRP, GRMP, SELP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, GMP-140, LECAM3, PADGEM

1 Images

Unknown

Western blot - Anti-P-Selectin (CD62P) antibody (AB59738)

All lanes:

Western blot - Anti-P-Selectin (CD62P) antibody (ab59738) at 0.5 µg/mL

All lanes:

Jurkat cell lysate

Predicted band size: 90 kDa

Observed band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Mouse Selp. The exact immunogen used to generate this antibody is proprietary information.

Q01102

Specificity

The antibody recognizes 84kDa CD62P.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3633R P-Selectin Antibody.

style

left-column

Properties and storage information

Form

Liquid

Purification notes

affinity purified

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62P also known as P-selectin is a cell adhesion molecule expressed on the surface of activated endothelial cells and platelets. It is a type of protein with a known molecular weight of approximately 140 kDa. P-selectin plays a mechanical role in mediating leukocyte rolling on the endothelium which is an initial step in the inflammatory response. This protein is rapidly translocated to the plasma membrane upon platelet activation and is stored in alpha granules of platelets and Weibel-Palade bodies of endothelial cells.

Biological function summary

P-selectin is critical in the recruitment of leukocytes to sites of tissue injury and infection. It facilitates the adhesion of leukocytes to the vascular endothelium by binding to its ligand PSGL-1 on the leukocyte surface. This interaction supports the transition of leukocytes from the circulation to the site of inflammation. P-selectin is also involved in thrombus formation as it contributes to the interactions between platelets leukocytes and endothelium in the vascular wall. It acts as part of a dynamic complex in the regulation of the innate immune response and hemostasis.

Pathways

CD62P plays an important role in both the inflammatory and coagulation pathways. It is an important mediator in the leukocyte adhesion cascade influencing leukocyte tethering and rolling on activated endothelium. The interaction of P-selectin with PSGL-1 initiates the signaling pathways that lead to firm adhesion and extravasation of leukocytes. In the coagulation pathway P-selectin enhances interactions between platelets and leukocytes which support thrombus formation. Its regulation and activity are tightly linked with other proteins such as E-selectin and L-selectin in coordinating vascular and systemic inflammatory responses.

Altered expression of CD62P is associated with various inflammatory conditions and cardiovascular diseases. In thrombosis increased levels of P-selectin enhance platelet aggregation and stability of the thrombus contributing to pathological clot formation. Similarly in atherosclerosis P-selectin facilitates the recruitment of monocytes to the vessel wall promoting plaque development. The protein's function relates to interactions with key inflammatory mediators and its dysregulation is often observed in conditions like chronic inflammatory diseases and acute myocardial infarction.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B : ITGB3) and integrin alpha-V/beta-3 (ITGAV : ITGB3) (PubMed : 37184585).

See full target information SELP

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106655 PubMed37168551

2023

Seizure-induced neutrophil adhesion in brain capillaries leads to a decrease in postictal cerebral blood flow.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Kyoung Lim,Sungjun Bae,Kayoung Han,Bok-Man Kang,Yoonyi Jeong,Seong-Gi Kim,Minah Suh

International journal of molecular sciences 23: PubMed36012397

2022

Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Jung Chen,Chih-Cheng Wu,Cheng-Yi Chang,Jian-Ri Li,Yen-Chuan Ou,Wen-Ying Chen,Su-Lan Liao,Jiaan-Der Wang

Cell metabolism 33:1793-1807.e9 PubMed34358431

2021

Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4 endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Guillermo Turiel,Raphaela Ardicoglu,Moheb Ghobrial,Evi Masschelein,Tea Kocijan,Jing Zhang,Ge Tan,Gillian Fitzgerald,Tatiane Gorski,Abdiel Alvarado-Diaz,Paola Gilardoni,Christopher M Adams,Bart Ghesquière,Katrien De Bock

Archives of biochemistry and biophysics 703:108846 PubMed33744198

2021

Thrombopoietic effects of CCAAT/enhancer-binding protein β on the early-stage differentiation of megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

HaiXu Song,Jiahao Liu,Xiaoxiang Tian,Dan Liu,Jiayin Li,Xiaojie Zhao,Zhu Mei,Chenghui Yan,Yaling Han

Vascular pharmacology 136:106808 PubMed33130016

2020

Platelet apelin receptor expression is reduced in patients with acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Strohbach,Andreas Böhm,Shailaja Mahajan-Thakur,Christopher Wirtz,Hanno Wetzel,Mathias C Busch,Stephan B Felix,Bernhard H Rauch,Raila Busch

PloS one 15:e0233725 PubMed32470058

2020

Long term effects of soluble endoglin and mild hypercholesterolemia in mice hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Barbora Vitverova,Iveta Najmanova,Matej Vicen,Katarina Tripska,Ivone Cristina Igreja Sa,Radek Hyspler,Miguel Pericacho,Petr Nachtigal

Atherosclerosis 283:19-27 PubMed30771557

2019

Inflammatory adipocyte-derived extracellular vesicles promote leukocyte attachment to vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Wadey,Katherine D Connolly,Donna Mathew,Gareth Walters,D Aled Rees,Philip E James

Diabetes 67:2668-2681 PubMed30213824

2018

Histone H3 Serine 10 Phosphorylation Facilitates Endothelial Activation in Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tamadher A Alghamdi,Sri N Batchu,Mitchell J Hadden,Veera Ganesh Yerra,Youan Liu,Bridgit B Bowskill,Suzanne L Advani,Laurette Geldenhuys,Ferhan S Siddiqi,Syamantak Majumder,Andrew Advani

American journal of physiology. Gastrointestinal a 292:G1173-80 PubMed17234893

2007

Treatment with BX471, a CC chemokine receptor 1 antagonist, attenuates systemic inflammatory response during sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Min He,Richard Horuk,Shabbir M Moochhala,Madhav Bhatia

American journal of physiology. Gastrointestinal and liver physiology 292:G1283-92 PubMed17218475

2007

Effect of CP-96,345 on the expression of adhesion molecules in acute pancreatitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hon Yen Lau,Madhav Bhatia

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-P-Selectin (CD62P) antibody

Western blot - Anti-P-Selectin (CD62P) antibody (AB59738)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise