AB217345

Anti-CD63 antibody [EPR21151]

Name: Anti-CD63 antibody [EPR21151] - macrophage marker (ab217345) | Abcam
Brand: Abcam Limited
SKU: AB217345
Price: 205 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(5 Reviews)

(241 Publications)

Anti-CD63 antibody [EPR21151] (ab217345) is a rabbit monoclonal antibody detecting CD63 in Western Blot, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 140 publications

View Alternative Names

CD63, CD63 antigen, Cd63

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (AB217345)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (AB217345)

Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (AB217345)

Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-CD63 antibody [EPR21151] (AB217345)

Exposure times : Lanes 1-3 : 3 minutes; Lane 4 : 5 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular masses observed are consistent with what has been described in the literature (PMID : 9890706; PMID : 9790070).

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

Lanes 1 - 3:

Western blot - Anti-CD63 antibody [EPR21151] (ab217345) at 1/2000 dilution

Lane 4:

Western blot - Anti-CD63 antibody [EPR21151] (ab217345) at 1/1000 dilution

Lane 1:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 40-60 kDa

true

Carrier free

Anti-CD63 antibody [EPR21151] - BSA and Azide free
660 APC

APC Anti-CD63 antibody [EPR21151]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD63 antibody [EPR21151]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD63 antibody [EPR21151]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD63 antibody [EPR21151]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD63 antibody [EPR21151]
578 PE

PE Anti-CD63 antibody [EPR21151]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21151

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

Product Specifications

Anti-CD63 antibody [EPR21151] (ab217345) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, WB in mouse samples.
Anti-CD63 antibody [EPR21151] (ab217345) specifically detects CD63 (UniProt ID: P41731; Molecular weight: 26kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD63 antibody [EPR21151] (ab217345) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD63 antibody [EPR21151] (ab217345) has been cited over 147 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD63 antibody [EPR21151] (ab217345) has 5 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR21151 - ab227892.
Antibody clone EPR21151 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, APC, PE, Alexa Fluor® 594, Alexa Fluor® 555 (ab233055, ab233056, ab319310, ab319449, ab319803, ab319944).
Target Information
CD63, also known as LAMP3 or Granulophysin, is a member of the tetraspanin family and is widely expressed on the surface of various cells, including macrophages and lymphocytes. It plays a crucial role in the formation and release of exosomes. CD63 (Granulophysin) is highly expressed in various tumor cells and also impacts the tumor microenvironment (TME) by regulating cell communication. Additionally, CD63 facilitates the degradation of the extracellular matrix, aiding in tumor cell invasion and metastasis.

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

See full target information CD63 antigen

style

left-column

Publications (241)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1629676 PubMed40995365

2025

Large extracellular vesicles derived from LPS-preconditioned cardiomyocytes alleviate myocarditis via mediating macrophage polarization and modulating p38 MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjie Jiang,Yingnan You,Yaxue Xie,Shan Zhou,Mengjie Ma,Bo Han

General physiology and biophysics 44:377-389 PubMed40923657

2025

Hypoxia-conditioned cardiomyocyte-derived exosomes attenuate myocardial injury via ANP-mediated M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Mingye Wang,Chi Zhao,Tongtong Li,Tao Song,Yuanyuan Hao,Wenwen Cui,Min Guan,Yunlong Hou,Yang Li

Redox biology 86:103826 PubMed40825268

2025

Macrophages and macrophage extracellular vesicles confer cancer ferroptosis resistance via PRDX6-mediated mitophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Naisheng Zheng,Fuli Li,Qing Huang,Xian Huang,Tomasz Maj

Journal of extracellular vesicles 14:e70114 PubMed40673783

2025

ADAM Sheddase Activity Promotes the Detachment of Small Extracellular Vesicles From the Plasma Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Chloé Bizingre,Zaira Arellano-Anaya,Flavien Picard,Mathéa Pietri,Anne Baudry,Florence Roussel,Clara Bianchi,Aurélie Alleaume-Butaux,Hector Ardila-Osorio,Maryse Romao,Grégory Lavieu,Graça Raposo,Benoit Schneider

General physiology and biophysics 44:337-348 PubMed40567076

2025

Exosomal microRNA-22-3p influences oxidative stress injury and neuronal apoptosis in Alzheimer's disease via targeting KDM6B.

Applications

Unspecified application

Species

Unspecified reactive species

Luzy Zhang

Cancers 17: PubMed40563579

2025

Comprehensive Profiling of Serum Exosomes by a Multi-Omics Approach Reveals Potential Diagnostic Markers for Brain Metastasis in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwoo Lim,Mia Kang,Young-Ho Ahn,Min-Sun Cho,Jin Hwa Lee,Jihee Lee Kang,Youn-Hee Choi

Journal of nanobiotechnology 23:439 PubMed40514650

2025

Nanoscale engineered exosomes for dual delivery of Sirtuin3 and insulin to ignite mitochondrial recovery in myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Xinyi Yun,Weihan Zheng,Huihui Zhang,Zi Yan,Youyu Chen,Wanting Xue,Siqi Mi,Ziyue Li,Hanxiao Sun,Guozhi Xiao,Zhenning Dai,Shiyu Li,Wenhua Huang

The Journal of cell biology 224: PubMed40488669

2025

Metabolic adaptations of micrometastases alter EV production to generate invasive microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Michalis Gounis,America V Campos,Engy Shokry,Louise Mitchell,Ruhi Deshmukh,Emmanuel Dornier,Nicholas Rooney,Sandeep Dhayade,Luis Pardo,Madeleine Moore,David Novo,Jenna Mowat,Craig Jamieson,Emily Kay,Sara Zanivan,Nikki R Paul,Claire Mitchell,Colin Nixon,Iain Macpherson,Saverio Tardito,David Sumpton,Karen Blyth,Jim C Norman,Cassie J Clarke

International journal of nanomedicine 20:6929-6948 PubMed40458745

2025

Proteomic Landscape of Small Extracellular Vesicles Derived From Gastric Juice and Identified TFF2 as a Specific Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiling Fan,Kaiwen Wu,Jingwei Yang,Binjun Zhu,Tao Jiang,Yu Liu,Bin Yuan,Lüye Liu,Yueshan Sun,Xiaobin Sun,Liu Liu,Wencai Luo,Chunyang Zhou,Yuanbiao Guo,Lei Liu

Journal of extracellular vesicles 14:e70100 PubMed40439163

2025

Glutamatergic Regulation of miRNA-Containing Intraluminal Vesicle Trafficking and Extracellular Vesicle Secretion From Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Marcela Bertolio,Qiyi Li,Francesca E Mowry,Kathryn E Reynolds,Rashed Alananzeh,Haichao Wei,Kyoeun Keum,Rachel Jarvis,Jiaqian Wu,Yongjie Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD63 antibody [EPR21151]

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (AB217345)

Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (AB217345)

Western blot - Anti-CD63 antibody [EPR21151] (AB217345)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (241)

Product promise