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AB325505

Anti-CD63 antibody [EPR29326-502] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD63 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD63, CD63 antigen, Cd63

5 Images
Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)

This data was developed using ab325495, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of bEnd.3 (mouse brain endothelial cell) cells labelling CD63 with ab325495 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody)/ Blue.

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)

This data was developed using ab325495, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) and NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD63 with ab325495 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody)/ Blue.

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control : EL4.

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)

This data was developed using ab325495, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte, Left) and RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD63 with ab325495 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody)/ Blue.

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control : A20.

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)

This data was developed using ab325495, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow cells labelling CD63 with ab325495 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD19 conjugated to PE/Cy7.

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD63 antibody [EPR29326-502] - BSA and Azide free (AB325505)

This data was developed using ab325495, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow cells labelling CD63 with ab325495 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29326-502

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab325505 is the carrier-free version of ab325495.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
See full target information CD63 antigen
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com