Anti-CD63 antibody - Late Endosome Marker (ab216130) is a rabbit polyclonal antibody that is used to detect CD63 in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 130 publications
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- Journal of translational medicine 21:9272023Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofang Zhou,Mu Liu,Lijuan Sun,Yumei Cao,Shanmei Tan,Guangxia Luo,Tingting Liu,Ying Yao,Wangli Xiao,Ziqing Wan,Jie TangPubMed 38129848
- Cell death & disease 14:8462023Mutual communication between radiosensitive and radioresistant esophageal cancer cells modulates their radiosensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongying Xie,Xiao Chen,Yueming Chen,Xingyue Wang,Jiwei Zuo,Anqi Zheng,Zhicheng Luo,Xiaoxiao Cheng,Shouhui Zhong,Jiayu Jiang,Jizao Du,Yuemei Zhao,Peipei Jiang,Wei Zhang,Didi Chen,Huanle Pan,Lanxiao Shen,Baoling Zhu,Qingyu Zhou,Yunsheng Xu,Kai-Fu TangPubMed 38114473
- Nutrients 15:2023Paeoniflorin Induces ER Stress-Mediated Apoptotic Cell Death by Generating Nox4-Derived ROS under Radiation in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Woo KimPubMed 38140352
- Cellular & molecular biology letters 28:922023Exosomal miR-155-5p drives widespread macrophage M1 polarization in hypervirulent Klebsiella pneumoniae-induced acute lung injury via the MSK1/p38-MAPK axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYihan Xu,Chunying Zhang,Danni Cai,Rongping Zhu,Yingping CaoPubMed 37953267
- Non-coding RNA 9:2023Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKseniia V Dracheva,Irina A Pobozheva,Kristina A Anisimova,Stanislav G Balandov,Maria N Grunina,Zarina M Hamid,Dmitriy I Vasilevsky,Sofya N Pchelina,Valentina V MiroshnikovaPubMed 37987363
- Respiratory research 24:2332023Mesenchymal stem cell-derived extracellular vesicles prevent the formation of pulmonary arterial hypertension through a microRNA-200b-dependent mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengzhi Wan,Caiju Lu,Yu Liu,Feng Luo,Jing Zhou,Fei XuPubMed 37759281
- Biomedicines 11:2023Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Lu,Xianwei Su,Lihong Wang,Chi-Kwan Leung,Jingye Zhou,Zhiqiang Xiong,Wuming Wang,Hongbin Liu,Wai-Yee ChanPubMed 37760991
- Diabetologia 66:2368-23862023Lipotoxicity-polarised macrophage-derived exosomes regulate mitochondrial fitness through Miro1-mediated mitophagy inhibition and contribute to type 2 diabetes development in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Ming Li,Xianyu Li,Lawrence W C Chan,Ruinian Hu,Tian Zheng,Haojie Li,Sijun YangPubMed 37615690
- Cell death & disease 14:4512023Exosomal circTGFBR2 promotes hepatocellular carcinoma progression via enhancing ATG5 mediated protective autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wang,Feng-Lin Dong,Ying-Qiao Wang,Hong-Long Wei,Tao Li,Jie LiPubMed 37474520
- International journal of molecular sciences 24:2023Fisetin, an Anti-Inflammatory Agent, Overcomes Radioresistance by Activating the PERK-ATF4-CHOP Axis in Liver Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae Woo KimPubMed 37240422
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD63. The exact immunogen used to generate this antibody is proprietary information. Database link P08962
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 - 1/2000 | Notes The observed molecular weight of CD63 may vary in the range of 25-65. This is due to a number of post translational modifications, post translation cleavages, relative charges, and other experimental factors, and therefore there really is no consensus molecular weight. |
Species Human | Dilution info 1/200 - 1/2000 | Notes The observed molecular weight of CD63 may vary in the range of 25-65. This is due to a number of post translational modifications, post translation cleavages, relative charges, and other experimental factors, and therefore there really is no consensus molecular weight. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
The protein expressed by gene CD63 functions as a cell surface receptor for TIMP1 and is involved in the activation of various cellular signaling cascades. It plays a role in the activation of ITGB1 and integrin signaling, which leads to the activation of AKT, FAK/PTK2, and MAP kinases, promoting cell survival, actin cytoskeleton reorganization, and cell adhesion, spreading, and migration through the activation of AKT and FAK/PTK2. Additionally, it participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2. CD63 is involved in intracellular vesicular transport processes and is essential for the normal trafficking of the PMEL luminal domain, crucial for melanocyte development and maturation. It also contributes to leukocyte adhesion onto endothelial cells by regulating SELP trafficking. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in response to other stimuli. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30
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Anti-CD63 antibody - Late Endosome Marker (ab216130) is a rabbit polyclonal antibody that is used to detect CD63 in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 130 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD63. The exact immunogen used to generate this antibody is proprietary information. Database link P08962
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD63 antibody - Late Endosome Marker (ab216130) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of CD63?
Anti-CD63 - Late Endosome Marker (ab216130) specifically detects a band for CD63 (UniProt: P08962) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-CD63 - Late Endosome Marker (ab216130) was first used in a scientific publication in 2016 and has been cited over 130 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
- Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
- Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
- Associated diseases and disorders
CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CD63 antibody - Late Endosome Marker (ab216130)
Reducing 12% SDS-PAGE
All lanes: Western blot - Anti-CD63 antibody - Late Endosome Marker (ab216130) at 1/200 dilution
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 40 µg
Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow cell lysate at 40 µg
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody - Late Endosome Marker (ab216130)
Paraffin-embedded human liver cancer tissue stained for CD63 with ab216130 at a 1/25 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
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