Anti-CD63 antibody - Late Endosome Marker
- Lab Essentials
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(202 Publications)
Anti-CD63 antibody - Late Endosome Marker (ab216130) is a rabbit polyclonal antibody detecting CD63 in Western Blot, IHC-P. Suitable for Human, Mouse.
- Over 130 publications
View Alternative Names
CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30
- WB
Supplier Data
Western blot - Anti-CD63 antibody - Late Endosome Marker (AB216130)
Reducing 12% SDS-PAGE
All lanes:
Western blot - Anti-CD63 antibody - Late Endosome Marker (ab216130) at 1/200 dilution
Lane 1:
NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 40 µg
Lane 2:
K562 (Human chronic myelogenous leukemia cell line from bone marrow cell lysate at 40 µg
Lane 3:
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 40 µg
Predicted band size: 26 kDa
true
Exposure time: 20s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody - Late Endosome Marker (AB216130)
Paraffin-embedded human liver cancer tissue stained for CD63 with ab216130 at a 1/25 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
Reactivity data
Product details
Anti-CD63 antibody - Late Endosome Marker (ab216130) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of CD63?
Anti-CD63 - Late Endosome Marker (ab216130) specifically detects a band for CD63 (UniProt: P08962) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-CD63 - Late Endosome Marker (ab216130) was first used in a scientific publication in 2016 and has been cited over 130 times in peer-reviewed journals.
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.
Pathways
CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.
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Target data
Publications (202)
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International journal of molecular sciences 26: PubMed41009720
2025
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Biology 14: PubMed40723426
2025
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Pharmaceutics 17: PubMed40284489
2025
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Scientific reports 15:9192 PubMed40097471
2025
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Antioxidants (Basel, Switzerland) 14: PubMed39857411
2025
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Stem cell research & therapy 15:493 PubMed39707562
2024
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ACS applied materials & interfaces 16:67235-67245 PubMed39582356
2024
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Biology 13: PubMed39336161
2024
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Frontiers in microbiology 15:1399632 PubMed39282564
2024
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Journal of nanobiotechnology 22:493 PubMed39160590
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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