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AB8219

Anti-CD63 antibody [MEM-259]

5

(8 Reviews)

|

(127 Publications)

Anti-CD63 antibody [MEM-259] (ab8219) is a mouse monoclonal antibody detecting CD63 in Flow Cytometry, IHC-P. Suitable for Human.

- Over 100 publications
- Trusted since 2001

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

8 Images
Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)
  • ICC

Unknown

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)

Immunocytochemistry staining of CD63 in human HeLa cell line using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)
  • ICC

Unknown

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)

Immunocytochemistry staining of human skin fibroblasts with ab8219 (green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)
  • ICC

Unknown

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)

Immunocytochemistry staining of CD63 in human primary fibroblasts using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (AB8219)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (AB8219)

Immunohistochemistry staining of human spleen (paraffin sections) using ab8219.

Flow Cytometry - Anti-CD63 antibody [MEM-259] (AB8219)
  • Flow Cyt

AbReview15198****

Flow Cytometry - Anti-CD63 antibody [MEM-259] (AB8219)

ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry - Anti-CD63 antibody [MEM-259] (AB8219)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD63 antibody [MEM-259] (AB8219)

Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml).

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)
  • ICC

AbReview78611****

Immunocytochemistry - Anti-CD63 antibody [MEM-259] (AB8219)

Immunocytochemistry analysis of acetone-fixed human melanoma cells staining with ab8219 at 1/500 dilution. Secondary antibody was FITC Anti mouse IgG at 1/200 dilution. Samples were incubated with the primary antibody PBS plus BSA for 1 hour. Blocking was done using 4% serum for 1 hour at room temperature.

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (AB8219)
  • IHC-P

AbReview78402****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MEM-259] (AB8219)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde fixed human melanoma tissue. Stained with ab8219 at 1/500 dilution. Secondary antibody used was Alkaline phosphatase Anti mouse/rabbit kit. Blocking was done with 4% serum for 30 minutes at 21°C. The sample was incubated with the primary antibody and Dako antibody diluent for 1 hour at 21°C. Antigen retrieval method was heat mediated with EDTA

This image is courtesy of an anonymous Abreview

  • 578 PE

    PE Anti-CD63 antibody [MEM-259]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD63 antibody [MEM-259]

  • 675 PerCP

    PerCP Anti-CD63 antibody [MEM-259]

  • Biotin

    Biotin Anti-CD63 antibody [MEM-259]

  • 519 FITC

    FITC Anti-CD63 antibody [MEM-259]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-259

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P, ICC

applications

Immunogen

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg/mL", "FlowCyt-species-notes": "<p>Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-CD63 antibody [MEM-259] (ab8219) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-CD63 [MEM-259] (ab8219) was first used in a scientific publication in 2001 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD63 [MEM-259] (ab8219) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [MEM-259] also available for your convenience: ab8219, FITC - ab18235, PE - ab18236, PerCP - ab77227, Biotin - ab134331, ab234251
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
See full target information CD63
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Publications (127)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23217 PubMed40603896

2025

Irradiation of prostate cancer alters circulating small extracellular vesicle functions.

Applications

Unspecified application

Species

Unspecified reactive species

Aejaz Sayeed,Vaughn Garcia,Cecilia E Verrillo,Rachel M DeRita,Md Niamat Hossain,Shiv Ram Krishn,Samuel Sey,Christopher D Shields,Adrian D Altieri,Qin Liu,Khalid Sossey-Alaoui,William K Kelly,Lucia R Languino

International journal of molecular sciences 26: PubMed40564943

2025

Flow Cytometric Quantification of Mitochondrial Properties: A High-Throughput Approach for Single Organelle Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J Piasecki,Hannah C Sheehan,Jonathan L Tilly,Dori C Woods

iScience 28:112538 PubMed40491474

2025

-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ngoc Vuong,Melany Alomia,Ahana Byne,Purva Gade,Thomas Raymond Philipson,Rayan Ibrahim Alhammad,Cade J Skislak,Intisar Alruwaili,Fahad M Alsaab,Weidong Zhou,Marissa Howard,Andrea Brothers,Amanda Haymond Still,Robyn P Araujo,Monique Van Hoek,Barbara Birkaya,Virginia Espina,Richard A Hoefer,Lance Liotta,Alessandra Luchini

PloS one 20:e0323529 PubMed40372993

2025

Characterization of small extracellular vesicles from ovarian cancer patients and pre-diagnostic patient samples: Evidence from the Danish blood donor study.

Applications

Unspecified application

Species

Unspecified reactive species

Nanna Lond Skov Frisk,Malene Møller Jørgensen,Rikke Bæk,Amila Iriskic Atic,Thorsten Rejkjær Brodersen,Sisse Rye Ostrowski,Margit Hørup Larsen,Dorthe Posselt,Estrid Høgdall,Claus Høgdall,Ole Birger Vesterager Pedersen,Louise Torp Dalgaard

Balkan medical journal 42:242-253 PubMed40326845

2025

Potential Roles of Serum Exosomal CD155 and its Impact on NK Cell Immunosuppression in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzheng Han,Jinrong Lv,Mintuo Wang,Xiaoxin Wu,Dongdong Sun,Wenwen Chen,Yingying Wang,Wenjie Zhou,Yuxuan Yang,Jia Bao,Qingzhen Han,Xiaopeng Chen,Fei Guo,Gang Feng,Min Li,Qing Chen

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed40300834

2025

The Role of Neprilysin and Insulin-Degrading Enzyme in the Etiology of Sporadic Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Morito,Shoko Hashimoto,Risa Takamura,Naoto Watamura,Naomasa Kakiya,Ryo Fujioka,Naomi Mihara,Misaki Sekiguchi,Kaori Watanabe-Iwata,Naoko Kamano,Mohan Qi,Yukio Matsuba,Satoshi Tsubuki,Takashi Saito,Nobuhisa Iwata,Hiroki Sasaguri,Takaomi C Saido

The Journal of clinical investigation 135: PubMed40111409

2025

Erythrocyte-derived extracellular vesicles induce endothelial dysfunction through arginase-1 and oxidative stress in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Collado,Rawan Humoud,Eftychia Kontidou,Maria Eldh,Jasmin Swaich,Allan Zhao,Jiangning Yang,Tong Jiao,Elena Domingo,Emelie Carlestål,Ali Mahdi,John Tengbom,Ákos Végvári,Qiaolin Deng,Michael Alvarsson,Susanne Gabrielsson,Per Eriksson,Zhichao Zhou,John Pernow

The Journal of biological chemistry 301:108305 PubMed39947471

2025

Integrin beta 1 and fibronectin mediate extracellular vesicle uptake and functional RNA delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Omnia M Elsharkasy,Willemijn S de Voogt,Maria Laura Tognoli,Leanne van der Werff,Jerney J Gitz-Francois,Cornelis W Seinen,Raymond M Schiffelers,Olivier G de Jong,Pieter Vader

Nature 632:1110-1117 PubMed39169179

2024

Lysosomes drive the piecemeal removal of mitochondrial inner membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Akriti Prashar,Claudio Bussi,Antony Fearns,Mariana I Capurro,Xiaodong Gao,Hiromi Sesaki,Maximiliano G Gutierrez,Nicola L Jones

Journal of extracellular vesicles 13:e12479 PubMed38978321

2024

Extracellular vesicle isolation and counting system (EVics) based on simultaneous tandem tangential flow filtration and large field-of-view light scattering.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Hyun Bae,Chan-Hyeong Lee,Dokyung Jung,Kyungmoo Yea,Byoung-Joon Song,Hakho Lee,Moon-Chang Baek
View all publications
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Product promise

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