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AB213090

Anti-CD63 antibody [NKI/C3] - BSA and Azide free

1

(1 Review)

|

(28 Publications)

Mouse Monoclonal CD63 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 28 publications. Immunogen corresponding to Cell preparation containing CD63 protein.

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

4 Images
Flow Cytometry - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)

Flow cytometry analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling CD63 using PE conjugated ab213090 (Red). Isotype control in Green; cells alone in black.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)

Immunohistochemical analysis of paraffin embedded, formalin fixed human melanoma tissue labeling CD63 with ab213090 at 1 μg/mL dilution. EAC chromogen labeling.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)

Immunohistochemical analysis of paraffin embedded, formalin fixed mouse spleen tissue labeling CD63 with ab213090 at 1 μg/mL dilution. DAB detection.

Western blot - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)
  • WB

Supplier Data

Western blot - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (AB213090)

All lanes:

Western blot - Anti-CD63 antibody [NKI/C3] - BSA and Azide free (ab213090) at 1 µg/mL

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 26 kDa

false

  • 578 PE

    PE Anti-CD63 antibody [NKI/C3] - Late Endosome Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NKI/C3

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.
Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
See full target information CD63

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:31 PubMed40016734

2025

Blocking the SIRPα-CD47 axis promotes macrophage phagocytosis of exosomes derived from visceral adipose tissue and improves inflammation and metabolism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Kai Lin,Yu-Fei Pan,Tian-Yi Jiang,Yi-Bin Chen,Tai-Yu Shang,Meng-You Xu,Hui-Bo Feng,Yun-Han Ma,Ye-Xiong Tan,Hong-Yang Wang,Li-Wei Dong

Lab on a chip 25:1718-1727 PubMed40007431

2025

Microfluidic loading of verteporfin into extracellular vesicles for neuroblastoma therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Piunti,Sara Micheli,Sara Giancaterino,Pina Fusco,Cristiana Boi,Elisa Cimetta

Aging 16:4609-4630 PubMed38428405

2024

Myogenic exosome miR-140-5p modulates skeletal muscle regeneration and injury repair by regulating muscle satellite cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Cao,Linli Xue,Xiuju Yu,Yi Yan,Jiayin Lu,Xiaomao Luo,Haidong Wang,Juan Wang

Heliyon 9:e19934 PubMed37809369

2023

BMP-7 modified exosomes derived from synovial mesenchymal stem cells attenuate osteoarthritis by M2 polarization of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Weixue Sun,Shaozheng Qu,Mingxia Ji,Yanli Sun,Baiqiang Hu

ASN neuro 15:17590914231183272 PubMed37345290

2023

Müller Glia to Müller Glia Extracellular Vesicle-Dependent Signaling Induces Multipotency Genes and l Expression in Response to N-methyl-D-aspartate (NMDA) Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Karen Carapia,Erick J Martinez-Colin,Deisy Segura-Villalobos,Rebeca Yael Victoria-Chavez,Ivonne Lezama,Eduardo Martinez-Martinez,Monica Lamas

Cell & bioscience 13:89 PubMed37202777

2023

miR-210-3p enriched extracellular vesicles from hypoxic neuroblastoma cells stimulate migration and invasion of target cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pina Fusco,Anna Fietta,Maria Rosaria Esposito,Luca Zanella,Sara Micheli,Angelica Bastianello,Lorenzo Bova,Giulia Borile,Giuseppe Germano,Elisa Cimetta

Cardiovascular toxicology 23:23-31 PubMed36609664

2023

Trophoblast Stem-Cell-Derived Exosomes Alleviate Cardiotoxicity of Doxorubicin via Improving Mfn2-Mediated Mitochondrial Fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Duan,Xiaoli Liu,Song Shen,Xi Tan,Yi Wang,Lian Wang,Lina Kang,Kun Wang,Zhonghai Wei,Yu Qi,Lei Hu,Biao Xu,Rong Gu

Cancers 14: PubMed36077635

2022

Platelet Activation in Ovarian Cancer Ascites: Assessment of GPIIb/IIIa and PF4 in Small Extracellular Vesicles by Nano-Flow Cytometry Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Bortot,Alessandro Mangogna,Ben Peacock,Rebecca Lees,Francesco Valle,Marco Brucale,Sara Tassinari,Federico Romano,Giuseppe Ricci,Stefania Biffi

Cell death & disease 13:725 PubMed35986010

2022

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Applications

Unspecified application

Species

Unspecified reactive species

Zixu Bao,Ning Zhang,Wanxiang Niu,Maolin Mu,Xiaoming Zhang,Shanshan Hu,Chaoshi Niu

Molecular & cellular toxicology :1-11 PubMed35967466

2022

Intestine epithelial cell-derived extracellular vesicles alleviate inflammation induced by TcdB through the activity of TGF-β1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuang Wan,Guangzhong Song,Hui Hu,Yaqing Xu,Peng Zeng,Shan Lin,Jun Yang,Jinqin Jiang,Xiaojun Song,Yongneng Luo,Dazhi Jin
View all publications

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