Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)

Rabbit Recombinant Multiclonal CD63 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt, IP and reacts with Human, Mouse samples.

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Images

Flow Cytometry - Anti-CD63 antibody [RM1095] - BSA and Azide free (AB315109), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IHC-P	Flow Cyt	IP
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information CD63 antigen

Function

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Additional Targets

CD63 antigen

Alternative names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

Recommended products

Rabbit Recombinant Multiclonal CD63 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P, Flow Cyt, IP and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1095
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse IHC, FC and IP.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315109 is the carrier-free version of Anti-CD63 antibody [RM1095] ab315108.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

Associated diseases and disorders

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Flow Cytometry - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / SK-MEL-28 (human malignant melanoma cell, Right) cells labelling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: Jurkat. Gated on viable cells.
Immunoprecipitation - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
CD63 was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma cell ) whole cell lysate with Anti-CD63 antibody [RM1095] ab315108 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD63 antibody [RM1095] ab315108 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SK-MEL-28 (human malignant melanoma cell ) whole cell lysate
Lane 2: Anti-CD63 antibody [RM1095] ab315108 IP in SK-MEL-28 (human malignant melanoma cell ) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD63 antibody [RM1095] ab315108 in SK-MEL-28 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CD63 antibody [RM1095] (Anti-CD63 antibody [RM1095] ab315108) at 1/30 dilution
All lanes: SK-MEL-28 (human malignant melanoma cell ) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
Western blot - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
CD63 is a glycoprotein of approximately 30-65 kDa and detected as 25kDa bands after de-glycosylation treatment.
Exposure time: Lanes 1-2: 37 seconds; Lanes 3-4: 180 seconds
All lanes: Western blot - Anti-CD63 antibody [RM1095] (Anti-CD63 antibody [RM1095] ab315108) at 1/1000 dilution
Lane 1: Untreated SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 15 µg
Lane 2: SK-MEL-28 whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 15 µg
Lane 3: Untreated bEnd.3 (mouse brain endothelial cell) whole cell lysate at 15 µg
Lane 4: bEnd.3 whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30-65 kDa, 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts myoblast) cells labelling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/100 (5.24 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in C2C12 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma) cells labelling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/100 (5.24 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in SK-MEL-28 cell line. Low expression cell line: Jurkat. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/5000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on the breast epithelial cells, positive staining on the stromal immune cells in human breast. The section was incubated with Anti-CD63 antibody [RM1095] ab315108 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/5000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on human skeletal muscle cells, but weak staining on the immune cells. The section was incubated with Anti-CD63 antibody [RM1095] ab315108 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/5000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil. The section was incubated with Anti-CD63 antibody [RM1095] ab315108 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling CD63 with Anti-CD63 antibody [RM1095] ab315108 at 1/5000 (0.105 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human melanoma. The section was incubated with Anti-CD63 antibody [RM1095] ab315108 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-CD63 antibody [RM1095] - BSA and Azide free (ab315109)
This data was developed using Anti-CD63 antibody [RM1095] ab315108, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Jurkat.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CD63 antibody [RM1095] (Anti-CD63 antibody [RM1095] ab315108) at 1/1000 dilution
Lane 1: SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg
Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 3: bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30-65 kDa, 36 kDa
Exposure time: 180s