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Mouse Monoclonal CD64 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing FCGR1_HUMAN protein.

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Images

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843), expandable thumbnail
  • Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843), expandable thumbnail
  • Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing FCGR1_HUMAN protein. The exact immunogen used to generate this antibody is proprietary information. Database link P12314

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-5.00000 µg/mL

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

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Target data

Function

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

Alternative names

Recommended products

Mouse Monoclonal CD64 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing FCGR1_HUMAN protein.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing FCGR1_HUMAN protein. The exact immunogen used to generate this antibody is proprietary information. Database link P12314
Clone number

10.1

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

ab119843 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.

Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

Associated diseases and disorders

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.

Product promise

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3 product images

  • Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843), expandable thumbnail

    Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)

    Surface staining of CD64 in human peripheral blood with anti-CD64 (10.1) purified , GAM-APC.

  • Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843), expandable thumbnail

    Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)

    Flow Cytometry analysis of human peripheral whole blood labeling CD64 positive monocytes with ab119843 at 4 μg/mL (red-filled).

    Negative control: CD64 negative lymphocytes (black-dashed).

  • Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843), expandable thumbnail

    Flow Cytometry - Anti-CD64 antibody [10.1] (ab119843)

    Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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