AB119843

Anti-CD64 antibody [10.1]

Name: Anti-CD64 antibody [10.1] (ab119843) | Abcam
Brand: Abcam Limited
SKU: AB119843
Price: 525 USD
Availability: InStock

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(10 Publications)

Mouse Monoclonal CD64 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing FCGR1A protein.

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

3 Images

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Flow Cytometry analysis of human peripheral whole blood labeling CD64 positive monocytes with ab119843 at 4 μg/mL (red-filled).

Negative control : CD64 negative lymphocytes (black-dashed).

Flow Cyt

Unknown

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Surface staining of CD64 in human peripheral blood with anti-CD64 (10.1) purified , GAM-APC.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10.1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing FCGR1A protein. The exact immunogen used to generate this antibody is proprietary information.

P12314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab119843 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.

Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

See full target information FCGR1A

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:2478 PubMed33510180

2021

Comparison of DNA repair and radiosensitivity of different blood cell populations.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Heylmann,Viviane Ponath,Thomas Kindler,Bernd Kaina

FASEB journal : official publication of the Federa 33:6551-6563 PubMed30794428

2019

C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/β-catenin and ERK signaling in streptozocin-induced diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Zhi-Yuan Shen,Ke Wang,Wei Li,Jing-Ming Shi,Ezra Kombo Osoro,Naeem Ullah,Yan Zhou,Shang-Rong Ji

Frontiers in immunology 9:2676 PubMed30515163

2018

IVIg and LPS Co-stimulation Induces IL-10 Production by Human Monocytes, Which Is Compromised by an FcγRIIA Disease-Associated Gene Variant.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa K Kozicky,Susan C Menzies,Zheng Yu Zhao,Tariq Vira,Kiera Harnden,Kwestan Safari,Kate L Del Bel,Stuart E Turvey,Laura M Sly

Aging cell 17:e12825 PubMed30094915

2018

A molecular signature for delayed graft function.

Applications

Unspecified application

Species

Unspecified reactive species

Dagmara McGuinness,Suhaib Mohammed,Laura Monaghan,Paul A Wilson,David B Kingsmore,Oliver Shapter,Karen S Stevenson,Shana M Coley,Luke Devey,Robert B Kirkpatrick,Paul G Shiels

Nature medicine 24:1234-1245 PubMed29892064

2018

The human heart contains distinct macrophage subsets with divergent origins and functions.

Applications

Unspecified application

Species

Unspecified reactive species

Geetika Bajpai,Caralin Schneider,Nicole Wong,Andrea Bredemeyer,Maarten Hulsmans,Matthias Nahrendorf,Slava Epelman,Daniel Kreisel,Yongjian Liu,Akinobu Itoh,Thirupura S Shankar,Craig H Selzman,Stavros G Drakos,Kory J Lavine

Hepatology communications 1:911-927 PubMed29159325

2017

Role of Aramchol in steatohepatitis and fibrosis in mice.

Applications

IHC-P

Species

Unspecified reactive species

Marta Iruarrizaga-Lejarreta,Marta Varela-Rey,David Fernández-Ramos,Ibon Martínez-Arranz,Teresa C Delgado,Jorge Simon,Virginia Gutiérrez-de Juan,Laura delaCruz-Villar,Mikel Azkargorta,José L Lavin,Rebeca Mayo,Sebastiaan M Van Liempd,Igor Aurrekoetxea,Xabier Buqué,Donatella Delle Cave,Arantza Peña,Juan Rodríguez-Cuesta,Ana M Aransay,Felix Elortza,Juan M Falcón-Pérez,Patricia Aspichueta,Liat Hayardeny,Mazen Noureddin,Arun J Sanyal,Cristina Alonso,Juan Anguita,María Luz Martínez-Chantar,Shelly C Lu,José M Mato

Journal of leukocyte biology 98:983-94 PubMed26216934

2015

Intravenous immunoglobulin skews macrophages to an anti-inflammatory, IL-10-producing activation state.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa K Kozicky,Zheng Yu Zhao,Susan C Menzies,Mario Fidanza,Gregor S D Reid,Kevin Wilhelmsen,Judith Hellman,Naomi Hotte,Karen L Madsen,Laura M Sly

International immunology 13:1571-81 PubMed11717198

2001

CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

C Sánchez-Torres,G S García-Romo,M A Cornejo-Cortés,A Rivas-Carvalho,G Sánchez-Schmitz

Clinical and experimental immunology 75:414-20 PubMed2495203

1989

The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma.

Applications

Unspecified application

Species

Unspecified reactive species

Y Jayaram,A M Buckle,N Hogg

European journal of immunology 17:1453-9 PubMed3500057

1987

The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.

Applications

Unspecified application

Species

Unspecified reactive species

G J Dougherty,Y Selvendran,S Murdoch,D G Palmer,N Hogg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD64 antibody [10.1]

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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