AB203349

Anti-CD64 antibody

Name: Anti-CD64 antibody (ab203349) | Abcam
Brand: Abcam Limited
SKU: AB203349
Price: 510 USD
Availability: InStock

(1 Review)

(3 Publications)

Rabbit Polyclonal CD64 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FCGR1A aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody (AB203349)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody (AB203349)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling CD64 with ab203349 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody (AB203349)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling CD64 with ab203349 at 1/200 dilution, followed by conjugation to the secondary antibody.

Supplier Data

Western blot - Anti-CD64 antibody (AB203349)

All lanes:

Western blot - Anti-CD64 antibody (ab203349) at 1/200 dilution

Lane 1:

Rat lung lysate

Lane 2:

Human colon carcinoma lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 43 kDa

Observed band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FCGR1A aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P12314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "(or 1/50 - 1/200 using a fluorescent secondary antibody)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.

Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

See full target information FCGR1A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40305352

2025

Melatonin MT1 Receptor Expression in Luminal Invasive Ductal Breast Carcinoma in Postmenopausal Women.

Applications

Unspecified application

Species

Unspecified reactive species

Leda Pistiolis,Sahar Alawieh,Thorhildur Halldorsdottir,Anikó Kovács,Roger Olofsson Bagge

Frontiers in immunology 15:1322256 PubMed38524127

2024

CD64 plays a key role in diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuqin Zhang,Liuhong Yuan,Zhenyu Tan,Huiyan Wu,Feier Chen,Junjie Huang,Pengjun Wang,Brett D Hambly,Shisan Bao,Kun Tao

The American journal of pathology 193:380-391 PubMed37003622

2023

Inflammation-Associated Lung Tissue Remodeling and Fibrosis in Morphine-Dependent SIV-Infected Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Divya T Chemparathy,Susmita Sil,Shannon Callen,Hitendra S Chand,Mohan Sopori,Todd A Wyatt,Arpan Acharya,Siddappa N Byrareddy,Howard S Fox,Shilpa Buch

View all publications

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