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AB140779

Anti-CD64 antibody [OTI3D3]

5

(3 Reviews)

|

(20 Publications)

Anti-CD64 antibody [OTI3D3] (ab140779) is a mouse monoclonal antibody detecting CD64 in Western Blot, IHC-P, ICC/IF. Suitable for African green monkey, Human.

- Over 10 publications

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (AB140779)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (AB140779)

Immunohistochemical analysis of paraffin embedded Human ovary adenocarcinoma tissue labelling CD64 with ab140779 at a 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (AB140779)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD64 antibody [OTI3D3] (AB140779)

Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling CD64 with ab140779 at a 1/150 dilution.

Western blot - Anti-CD64 antibody [OTI3D3] (AB140779)
  • WB

Unknown

Western blot - Anti-CD64 antibody [OTI3D3] (AB140779)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210076)

All lanes:

Western blot - Anti-CD64 antibody [OTI3D3] (ab140779) at 1/4000 dilution

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY CD64 cDNA at 5 µg

Predicted band size: 43 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (AB140779)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD64 antibody [OTI3D3] (AB140779)

Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD64 using ab140779 at a 1/100 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3D3

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FCGR1A.

P12314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-CD64 antibody [OTI3D3] (ab140779) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human samples.

What is the molecular weight of CD64?
Anti-CD64 [OTI3D3] (ab140779) specifically detects a band for CD64 (UniProt: P12314) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-CD64 [OTI3D3] (ab140779) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [OTI3D3] also available for your convenience: ab140779, Carrier free - ab308144
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.
Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
See full target information FCGR1A
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102041 PubMed40168999

2025

TIMEPOINT, a phase 1 study combining MTL-CEBPA with pembrolizumab, supports the immunomodulatory effect of MTL-CEBPA in solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth Plummer,Mikael H Sodergren,Rose Hodgson,Bríd M Ryan,Nina Raulf,Joanna P Nicholls,Vikash Reebye,Jon Voutila,Laura Sinigaglia,Tim Meyer,David J Pinato,Debashis Sarker,Bristi Basu,Sarah Blagden,Natalie Cook,Thomas R Jeffrey Evans,Jeffrey Yachnin,Cheng E Chee,Daneng Li,Anthony El-Khoueiry,Maria Diab,Kai-Wen Huang,Madhava Pai,Duncan Spalding,Thomas Talbot,Marcus S Noel,Bridget Keenan,Devalingam Mahalingam,Min-Sun Song,Mélanie Grosso,Denis Arnaud,Aurelie Auguste,Dimitris Zacharoulis,Jan Storkholm,Iain McNeish,Robert Habib,John J Rossi,Nagy A Habib

Journal of translational medicine 23:61 PubMed39806464

2025

The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Yan Yang,Nian Chen,Qian Wen,Yu Zhou,Tao Zhang,Ji Zhou,Cheng-Hui Liang,Li-Ping Han,Xiao-Ya Wang,Qing-Mei Kang,Xiao-Xia Zheng,Xue-Jia Zhai,Hong-Ying Jiang,Tian-Hua Shen,Jin-Wei Xiao,Yu-Xin Zou,Yun Deng,Shuang Lin,Jiang-Jie Duan,Jun Wang,Shi-Cang Yu

Chinese medical journal 137:1316-1323 PubMed38738696

2024

Development and validation of a novel criterion of histologic healing in ulcerative colitis defined by inflammatory cell enumeration in lamina propria mucosa: A multicenter retrospective cohort in China.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gao,Kangsheng Peng,Yadi Shi,Shenshen Zhu,Ruicong Sun,Chunjin Xu,Ping Liu,Zhi Pang,Lanxiang Zhu,Weichang Chen,Baisui Feng,Huili Wu,Guangxi Zhou,Mingsong Li,Junxiang Li,Baijing Ding,Zhanju Liu

Journal of translational medicine 21:793 PubMed37940996

2023

Muc2 mucin O-glycosylation interacts with enteropathogenic Escherichia coli to influence the development of ulcerative colitis based on the NF-kB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wei,Chunyan Chen,Jing Feng,Shuping Zhou,Xiaoyue Feng,Zhao Yang,Heng Lu,Hui Tao,Liuying Li,Huabing Xv,Ji Xuan,Fangyu Wang

BMC medical genomics 16:227 PubMed37759229

2023

Establishment and validation of an immune infiltration predictive model for ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxia Song,Jingwen Zhang,Yue Sun,Zhongmin Jiang,Xiaoning Liu

Aging 15:9822-9841 PubMed37737713

2023

Single-cell and bulk sequencing analyses reveal the immune suppressive role of PTPN6 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Jie Chen,Ming Zhang,Saisai Liu,Tao Wang,Tianyu Wu,Baiqing Li,Shidi Zhao,Hongtao Wang,Li Li,Chun Wang,Li Huang

iScience 26:107585 PubMed37694144

2023

Multi-omic approach identifies hypoxic tumor-associated myeloid cells that drive immunobiology of high-risk pediatric ependymoma.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea M Griesinger,Kent Riemondy,Nithyashri Eswaran,Andrew M Donson,Nicholas Willard,Eric W Prince,Simon M L Paine,Georgia Bowes,John Rheaume,Rebecca J Chapman,Judith Ramage,Andrew Jackson,Richard G Grundy,Nicholas K Foreman,Timothy A Ritzmann

Frontiers in immunology 14:1133886 PubMed37033941

2023

Human seminal plasma stimulates the migration of CD11c+ mononuclear phagocytes to the apical side of the colonic epithelium without altering the junctional complexes in an human intestinal model.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Baratella,Valeria Iannone,Mariangela Cavarelli,Chiara Foglieni,Paola Viganò,Christiane Moog,Ugo Elmore,Silvia Nozza,Massimo Alfano,Andrea Salonia,Stefania Dispinseri,Gabriella Scarlatti

Biomarker research 11:25 PubMed36879284

2023

Co-enrichment of CD8-positive T cells and macrophages is associated with clinical benefit of tislelizumab in solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Dingwei Ye,Jayesh Desai,Jingwen Shi,Si-Yang Maggie Liu,Wei Shen,Tengfei Liu,Yang Shi,Dan Wang,Liang Liang,Silu Yang,Xiaopeng Ma,Wei Jin,Pei Zhang,Ruiqi Huang,Zhirong Shen,Yun Zhang,Yi-Long Wu

Investigative ophthalmology & visual science 63:19 PubMed35731510

2022

Sleep Loss Causes Dysfunction in Murine Extraorbital Lacrimal Glands.

Applications

Unspecified application

Species

Unspecified reactive species

Shenzhen Huang,Hongli Si,Jiangman Liu,Di Qi,Xiaoting Pei,Dingli Lu,Sen Zou,Zhijie Li
View all publications
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