JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197678

Anti-CD66b antibody

Be the first to review this product! Submit a review

|

(38 Publications)

Rabbit Polyclonal CD66b antibody. Suitable for IHC-P and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEACAM8.

View Alternative Names

CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (AB197678)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (AB197678)

Immunohistochemical analysis of formalin fixed paraffin embedded human gastric cancer tissue labeling CD66b with ab197678 at a 1/30 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CEACAM8. The exact immunogen used to generate this antibody is proprietary information.

P31997

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>The method we used for antigen retrieval is heat induced epitope retrieval, and 10 mM sodium citrate buffer (pH 6) are used for heat-mediated retrieval by a pressure cooker. Once boiling, put the slides to the cooker. As soon as the cooker has reached full pressure, time 4 min.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD66b also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) is a glycoprotein with a molecular weight around 95 kDa. It is primarily expressed on the surface of granulocytes specifically on neutrophils—its expression on these cells makes it a useful marker in distinguishing stages of granulocyte maturation. The protein localizes to the cell membrane and serves as a cell adhesion molecule playing roles in intercellular interactions. Researchers often use CD66b-specific antibodies such as CD66b FITC for detecting and analyzing neutrophil populations commonly in flow cytometry applications.
Biological function summary

CD66b contributes to immune responses by facilitating leukocyte activation and migration during inflammation. It does not form part of a multi-protein complex but interacts with other cellular components. CD66b promotes neutrophil adhesion and transmigration to sites of infection or injury supporting their role in innate immunity. Additionally it participates in signaling pathways that influence cell survival proliferation and apoptosis in response to environmental cues.

Pathways

CD66b operates within neutrophil-related processes like the immune response and cell adhesion pathways. In the immune response pathway it collaborates with integrins to assist neutrophil extravasation through the endothelium. During inflammatory responses CD66b interaction with integrins and other adhesion molecules influences the intensity and duration of activity at inflammation sites. This interaction highlights its involvement with proteins such as ICAM-1 in mediating firm adhesion necessary for neutrophil migration.

CD66b levels and activity correlate with conditions involving inflammation such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. During COPD the altered expression and regulation of CD66b contribute to neutrophil-driven inflammation exacerbating disease symptoms. Similarly rheumatoid arthritis involves inflammatory pathways where aberrant CD66b activity enhances synovial inflammation often linked with other inflammatory markers like TNF-alpha. Understanding CD66b's role in such diseases aids the development of therapeutic strategies targeting neutrophil-mediated pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764).
See full target information CEACAM8
style
left-column

Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:106 PubMed39932547

2025

Neutrophil-induced pyroptosis promotes survival in patients with hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingyi Fu,Jiquan Zhou,Liyuan Yang,Jing Wang,Hui Li,Yuhua Shan,Hongxiang Gao,Chenjie Xie,Dapeng Jiang,Lei Zhang,Ji Ma,Qiuhui Pan,Min Xu,Min Zhang,Song Gu

Signal transduction and targeted therapy 9:342 PubMed39638994

2024

CXCR1 neutrophil infiltration orchestrates response to third-generation EGFR-TKI in EGFR mutant non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haowei Wang,Anwen Xiong,Xiaoxia Chen,Junhong Guo,Zhuoran Tang,Chunyan Wu,Shengxiang Ren,Caicun Zhou,Jian Chen,Likun Hou,Tao Jiang

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4601-4608 PubMed39167623

2024

Pembrolizumab and Cabozantinib in Recurrent and/or Metastatic Head and Neck Squamous Cell Carcinoma: Long-term Survival Update with a Biomarker Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nabil F Saba,Ritu Chaudhary,Kedar Kirtane,Angelo Marra,Asari Ekpenyong,Ashley McCook-Veal,Nicole C Schmitt,Jennifer H Gross,Mihir R Patel,Jill Remick,James E Bates,Mark W McDonald,Soumon F Rudra,William A Stokes,Maria Biernacki,Xiaofei Song,Robbert J C Slebos,Yuan Liu,Conor E Steuer,Dong M Shin,Yong Teng,Christine H Chung

JCI insight 9: PubMed38900571

2024

Intraepithelial CD15 infiltration identifies high-grade anal dysplasia in people with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Joaquín Burgos,Aleix Benítez-Martínez,Cristina Mancebo,Núria Massana,Antonio Astorga-Gamaza,Josep Castellvi,Stefania Landolfi,Adrià Curran,Jorge N Garcia-Perez,Vicenç Falcó,María J Buzón,Meritxell Genescà

Experimental hematology & oncology 13:58 PubMed38822440

2024

Multi-model analysis of gallbladder cancer reveals the role of OxLDL-absorbing neutrophils in promoting liver invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Dongning Rao,Jiaxin Li,Mao Zhang,Siyuan Huang,Lu Meng,Guohe Song,Jiaqiang Ma,Yingcheng Wu,Yifei Cheng,Shuyi Ji,Gaohua Wu,Lv Chen,Yuming Liu,Yang Shi,Jian Zhou,Fan Jia,Xiaoming Zhang,Ruibin Xi,Qiang Gao

BMC cancer 24:327 PubMed38462640

2024

The relationship between tumor-infiltrating neutrophils and clinical outcomes in patients with resectable hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hee Lee,Young Mi Hong

Clinica chimica acta; international journal of clinical chemistry 554:117773 PubMed38199579

2024

Exosomal miRNAs from neutrophils act as accurate biomarkers for gastric cancer diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yu,Jiahui Zhang,Maoye Wang,Runbi Ji,Hui Qian,Wenrong Xu,Hongbo Zhang,Jianmei Gu,Xu Zhang

Thrombosis research 234:1-8 PubMed38113606

2023

White blood cell subtypes and neutrophil extracellular traps content as biomarkers for stroke etiology in acute ischemic stroke clots retrieved by mechanical thrombectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Duaa Jabrah,Rosanna Rossi,Sara Molina,Andrew Douglas,Abhay Pandit,Ray McCarthy,Michael Gilvarry,Eric Ceder,Seán Fitzgerald,Dennis Dunker,Annika Nordanstig,Petra Redfors,Turgut Tatlisumak,Alan O'Hare,Sarah Power,Paul Brennan,Peter Owens,András Nagy,Ágnes Vadász,Simon F De Meyer,Georgios Tsivgoulis,Klearchos Psychogios,Istvan Szikora,Katarina Jood,Alexandros Rentzos,John Thornton,Karen Doyle

Physiological genomics 56:32-47 PubMed37955337

2023

Exploring myometrial microenvironment changes at the single-cell level from nonpregnant to term pregnant states.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyuan Ji,Junmin Zhong,Long Cui,Xiaodi Wang,Li-Na Chen,Bolun Wen,Fan Yang,Wenfeng Deng,Xiuyu Pan,Lele Wang,Junjie Bao,YunShan Chen,Huishu Liu

Journal of experimental & clinical cancer research : CR 42:236 PubMed37684625

2023

Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehua Kong,Yu Zhang,Linwei Xiang,Yan You,Yaqian Duan,Yuqing Zhao,Shue Li,Rui Wu,Jiangbo Zhang,Lan Zhou,Liang Duan
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB48589

Anti-CD66b antibody [GM-2H6]

Flow Cytometry - Anti-CD66b antibody [GM-2H6] (AB48589)

  • 0
  • 0
Primary Antibodies

AB233811

Anti-CD66b antibody [2F12E8]

Western blot - Anti-CD66b antibody [2F12E8] (AB233811)

  • 0
  • 0
Primary Antibodies

AB218740

Anti-CD66b antibody - BSA and Azide free

  • 0
  • 0
Primary Antibodies

AB214175

Anti-CD66b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody (AB214175)

  • 0
  • 0
Primary Antibodies

AB170875

Anti-CD66b antibody [EPR7701]

RabMAb|Recombinant

Western blot - Anti-CD66b antibody [EPR7701] (AB170875)

  • 0
  • 0

Complementary Products

Assay Kits

AB282869

Human CD66b ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)

  • 0
  • 0
Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

BOND RX™ Validated|Recombinant|Lab Essentials|Advanced Validation

Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] (AB20034)

  • 4
  • 38
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com