AB201973

Anti-CD68 antibody [3F7D3]

Lab Essentials
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(18 Publications)

Mouse Monoclonal CD68 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD68 aa 1-200.

View Alternative Names

CD68, Macrosialin, Gp110

7 Images

Flow Cytometry - Anti-CD68 antibody [3F7D3] (AB201973)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD68 antibody [3F7D3] (AB201973)

Flow cytometric analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green) compared to a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [3F7D3] (AB201973)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [3F7D3] (AB201973)

Immunofluorescent analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (AB201973)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (AB201973)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (AB201973)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.

Supplier Data

Western blot - Anti-CD68 antibody [3F7D3] (AB201973)

All lanes:

Western blot - Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 32 kDa,34 kDa,37 kDa,53 kDa,56 kDa,57 kDa,59 kDa,60 kDa,70 kDa,71 kDa,98 kDa

false

Supplier Data

Western blot - Anti-CD68 antibody [3F7D3] (AB201973)

Expected MWt is 37.4 kDa.

All lanes:

Western blot - Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

All lanes:

Human CD68 (aa42-155) recombinant protein

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-CD68 antibody [3F7D3] (AB201973)

All lanes:

Western blot - Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

Lane 1:

U937 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

Jurkat cell lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F7D3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CD68 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P34810

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR255595-19 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information CD68

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cell division 20:22 PubMed41039618

2025

FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

Nature communications 16:1871 PubMed39984440

2025

Activation of CAMK2 by pseudokinase PEAK1 represents a targetable pathway in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Xiuquan Ma,Tianyue Zhao,David R Croucher,Elizabeth V Nguyen,Kimberley C Clark,Changyuan Hu,Sharissa L Latham,Charles Bayly-Jones,Bao V Nguyen,Srikanth Budnar,Sung-Young Shin,Lan K Nguyen,Thomas R Cotton,Anderly C Chüeh,Terry C C Lim Kam Sian,Margaret M Stratton,Andrew M Ellisdon,Roger J Daly

Cell biology international 49:494-507 PubMed39949174

2025

Macrophage-to-Myofibroblast Transition Contributes to Cutaneous Scarring Formation Through the TGF-β/Smad3 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Jia,Yi Qin,Feng-Lai Yuan,Jie-Hong Shen

Naunyn-Schmiedeberg's archives of pharmacology 397:7187-7200 PubMed38652276

2024

The thrombin receptor PAR4 supports visceral adipose tissue inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja Kleeschulte,Vivien Fischinger,Lisa Öhlke,Johannes Bode,Markus Kamler,Dobromir Dobrev,Maria Grandoch,Anke C Fender

Cells 13: PubMed38247800

2024

A High-Protein Diet Promotes Atrial Arrhythmogenesis via Absent-in-Melanoma 2 Inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Song,Jiao Wu,Dexter J Robichaux,Tingting Li,Shuyue Wang,Maria J Arredondo Sancristobal,Bingning Dong,Dobromir Dobrev,Jason Karch,Sandhya S Thomas,Na Li

The Journal of biological chemistry 300:105641 PubMed38211816

2024

Disruption of lysosomal nutrient sensing scaffold contributes to pathogenesis of a fatal neurodegenerative lysosomal storage disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria B Bagh,Abhilash P Appu,Tamal Sadhukhan,Avisek Mondal,Nisha Plavelil,Mahadevan Raghavankutty,Ajayan M Supran,Sriparna Sadhukhan,Aiyi Liu,Anil B Mukherjee

International journal of environmental research and public health 20: PubMed37444145

2023

Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra D Goudreau,Layli Tanara,Velislava Tzaneva,Kristi B Adamo

Journal of digestive diseases 24:231-242 PubMed37155188

2023

IL-26 modulates T cell function in autoimmune hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei He,Qi Wei Qian,Qiao Yan Liu,Yi Kang Li,Qi Miao,Qi Xia Wang,Xiong Ma,Zheng Rui You,Ru Qi Tang

Physiological reports 11:e15741 PubMed37269190

2023

Characterization of Hofbauer cell polarization and VEGF localization in human term placenta from active and inactive pregnant individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra D Goudreau,Catherine Everest,Layli Tanara,Velislava Tzaneva,Kristi B Adamo

Cell death & disease 13:891 PubMed36270983

2022

NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinrui Rao,Xiaoshu Zhou,Geng Wang,Xiaohua Jie,Biyuan Xing,Yingzhuo Xu,Yunshang Chen,Jun Li,Kuikui Zhu,Zilong Wu,Gang Wu,Chuangyan Wu,Rui Zhou

View all publications

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